Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.
Used in the production of penicillin.
Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.
Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.
Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.
One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Lyophilized powder containing phosphate and citrate buffer salts
Protein determined by biuret.