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P0389

Sigma-Aldrich

Penicillinase from Bacillus cereus

lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

Synonym(s):
Penicillin amido-β-lactam hydrolase, β-Lactamase II, Cephalosporinase, β-Lactamase I from Bacillus cereus
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

Quality Level

form

lyophilized powder

specific activity

1,500-3,000 units/mg protein (using benzylpenicillin)

composition

Protein, ~10%

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

2-8°C

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General description

Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.

Application

Used in the production of penicillin.
Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.
Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Analysis Note

Protein determined by biuret.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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