P0501

Sigma-Aldrich

Phenolphthalein β-D-glucuronide

β-glucuronidase substrate

Synonym(s):
Phenolphthalein glucuronic acid, Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein mono-β-D-glucosiduronic acid
Empirical Formula (Hill Notation):
C26H22O10
CAS Number:
Molecular Weight:
494.45
Beilstein/REAXYS Number:
68526
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥97% (TLC)

form

powder

solubility

H2O: soluble 100 mg/mL

storage temp.

−20°C

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

Looking for similar products? Visit Product Comparison Guide

Application

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.
Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

Packaging

25, 100, 500 mg in poly bottle

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Effects of two pomace insoluble fibres on the activities of faecal bacterial enzymes and intestinal health
Chau CF and Chen CH
European Food Research and Technology, 222(5-6), 681-685 (2006)
A microtitre plate assay for measuring glycosidase activity
Ball Al, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 23(1), 131-135 (2008)
Improvement in intestinal function of hamsters as influenced by consumption of polysaccharide-rich sage weed extracts
Huang YL and Chau CF
Food Chemistry, 133(4), 1618-1623 (2012)
Intestinal health benefits of the water-soluble carbohydrate concentrate of wild grape (Vitis thunbergii) in hamsters
Huang YL, et al.
Journal of Agricultural and Food Chemistry, 60(19), 4854-4858 (2012)
Improvement in intestinal function and health by the peel fiber derived from Citrus sinensis L cv Liucheng
Chi-Fai Chau, Fuu Sheu, Ya-Ling Huang and Li-Hung Su
Journal of the Science of Food and Agriculture, 85, 1211-1216 (2005)
Articles
Drug conjugate analysis and the enzymatic hydrolysis of glucuronides
Read More
Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.
Read More
Protocols
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service