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P0623

Sigma-Aldrich

Monoclonal Anti-PRMT4 antibody produced in mouse

clone PRMT4-243, ascites fluid, buffered aqueous solution

Synonym(s):
Anti-Protein Arginine Methyl Transferase 4
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PRMT4-243, monoclonal

form

buffered aqueous solution

mol wt

antigen ~66 kDa

contains

15 mM sodium azide

species reactivity

rat, canine, monkey, bovine, human, mouse

technique(s)

western blot: 1:2,000-1:4,000 μg/mL using whole cell extract of 293T cells

isotype

IgG3

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CARM1(10498)

General description

Monoclonal Anti-PRMT4 (protein arginine methyl transferase 4) (mouse IgG3 isotype) is derived from the PRMT4-243 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. PRMTs are divided into two major types, type I and type II. PRMT4 is also referred as CARM1. This is a type I protein arginine methyltransferase (PRMT), that is located on human chromosome 19p13.2.

Specificity

Monoclonal Anti-PRMT4 recognizes human, monkey, bovine, canine, rat, and mouse PRMT4. The antibody does not cross react with PRMT 1, 2, 3, 5, and 6.

Immunogen

synthetic peptide corresponding to amino acids 48-63 of human PRMT4, conjugated to KLH.

Application

Monoclonal Anti-PRMT4 antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Protein arginine methyl transferase 4 (PRMT4) plays a central role in regulation of transcription by nuclear hormone receptors (NRs). Antibodies specific for PRMT4 (CARM1) are an important tool for studying the biology of PRMTs. Overexpression of coactivator-associated arginine methyltransferase 1 (CARM1) is observed in human cancers. It promotes the development of breast cancer. In hematopoietic cell lines, PRMT4 serves as a coactivator of c-Myb-dependent transcription. CARM1, which acts as an oncogene in cancers can asymmetrically dimethylates protein substrates on arginine residues.

Physical form

Monoclonal Anti-PRMT4 is supplied as ascites fluid with 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

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Certificate of Origin

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