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P0762

Sigma-Aldrich

Phosphatase, Alkaline−Agarose from calf intestine

ammonium sulfate suspension

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Synonym(s):
Alkaline phosphatase-Agarose
MDL number:
eCl@ss:
42010105

biological source

bovine (calf) intestine

Quality Level

form

ammonium sulfate suspension

specific activity

250-1000 units/mL packed gel

matrix

beaded agarose

storage temp.

2-8°C

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This Item
A2080P677410567752001
Alkaline Phosphatase EIA grade, from calf intestine

Roche

10567752001

Alkaline Phosphatase

form

ammonium sulfate suspension

form

PBS suspension

form

buffered aqueous solution

form

solution

specific activity

250-1000 units/mL packed gel

specific activity

-

specific activity

≥2,000 DEA units/mg protein

specific activity

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

matrix

beaded agarose

matrix

-

matrix

-

matrix

-

General description

Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000.

Application

Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine.
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases.

Biochem/physiol Actions

Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.

Packaging

Package size based on DEA units

Unit Definition

One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.

Physical form

Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets
Beumer, C.
Journal of Pharmacology and Experimental Therapeutics, 397, 737-744 (2003)
M Kellerer et al.
Diabetologia, 41(7), 833-838 (1998-08-01)
Protein kinase C (PKC) isoforms are potentially important as modulators of the insulin signalling chain and could be involved in the pathogenesis of cellular insulin resistance. We have previously shown that phorbol ester stimulated PKC beta1 and beta2 as well
Li-Na Li et al.
Guang pu xue yu guang pu fen xi = Guang pu, 29(10), 2820-2823 (2009-12-30)
To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
J E Coleman
Annual review of biophysics and biomolecular structure, 21, 441-483 (1992-01-01)
Alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two Zn ions and one Mg ion) are present at the active center. Zn ions at all three sites also produce a maximally
Rebika Shrestha et al.
iScience, 25(1), 103608-103608 (2022-02-03)
Understanding the spatiotemporal distribution and dynamics of RAS on the plasma membrane (PM) is the key for elucidating the molecular mechanisms of the RAS signaling pathway. Single particle tracking (SPT) experiments show that in cells, KRAS diffuses in at least

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