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P0913

Sigma-Aldrich

Polyinosinic–polycytidylic acid sodium salt

γ-irradiated

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Synonym(s):
Poly (I:C), Poly(I) • Poly(C)
CAS Number:
MDL number:
NACRES:
NA.51

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

Assay

≥99% (less than 1% free nucleotides, TLC)

form

lyophilized powder

storage condition

desiccated

solubility

H2O: 10 mg/mL

storage temp.

−20°C

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This Item
P1530P9582P4154
assay

≥99% (less than 1% free nucleotides, TLC)

assay

≥99% (less than 1% free nucleotides, TLC)

assay

≥99% (less than 1% free nucleotides, TLC)

assay

≥98% (TLC)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

solubility

H2O: 10 mg/mL

solubility

H2O: 10 mg/mL

solubility

-

solubility

water: 4.80-5.20 mg/mL, clear to hazy, colorless to faintly yellow

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Other Notes

Double-stranded homopolymer.

Preparation Note

The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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