P1107

Sigma-Aldrich

JumpStart REDTaq® ReadyMix Reaction Mix

for High-throughput PCR of complex templates

MDL number:
NACRES:
NA.55
Pricing and availability is not currently available.

Quality Level

form

liquid

feature

hotstart

concentration

0.75 units/reaction (50 μL reaction volume)

color

red

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.
JumpStart REDTaq ReadyMix PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL.

JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored.

The red dye allows quick visual confirmation the enzyme has been added and properly mixed. After PCR, an aliquot may be loaded directly onto an agarose gel without the addition of loading buffers. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired.

Using JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.

Application

  • For multiplex PCR
  • For amplification with reduced non-specific amplification
  • For high throughput PCR amplification of complex templates

Features and Benefits

  • JumpStart Taq DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
  • REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
  • Inert red dye allows for easy verification that reagent has been mixed properly
  • Other loading dyes not necessary. Aliquots may be directly loaded onto an agarose gel after the reaction

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Pictograms

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Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

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Articles
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
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Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
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Protocols
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
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Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
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JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
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