P1167

Pituitary Extract bovine

≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

• Synonym(s): BPE, PEX
• MDL number: MFCD00163156
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• Product Name: Pituitary Extract bovine, lyophilized powder, suitable for cell culture
• biological source: bovine
• Quality Level: 200
• form: lyophilized powder
• potency: 15 - 200 μg/mL EC₅₀
• quality: endotoxin tested
• packaging: pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤50 EU/mg
• color: brown
• solubility: 0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange
• storage temp.: 2-8°C

Description

General description

Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.[1]

Application

Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells.[2] It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.[3]

Biochem/physiol Actions

Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity[1] and promotes the growth of several types of mammalian cells.[4] It favors the proliferation of keratocytes culture[1] and bovine knee chondrocytes.[4] BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.[4]

Physical form

Lyophilized from sodium phosphate buffer