P1167

Pituitary Extract bovine

lyophilized powder, suitable for cell culture

• Synonym(s): BPE, PEX
• MDL number: MFCD00163156

Properties

• biological source: bovine
• Quality Level: 200
• form: lyophilized powder
• potency: 15 - 200 μg/mL EC₅₀
• quality: endotoxin tested
• packaging: pkg of 5 mg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤50 EU/mg
• color: brown
• solubility: 0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange
• storage temp.: 2-8°C

Description

General description

Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.

Application

Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells. It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.

Biochem/physiol Actions

Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity and promotes the growth of several types of mammalian cells. It favors the proliferation of keratocytes culture and bovine knee chondrocytes. BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.

Physical form

Lyophilized from sodium phosphate buffer