P1167

Sigma-Aldrich

Pituitary Extract bovine

lyophilized powder, suitable for cell culture

Synonym(s):
BPE, PEX
MDL number:

Quality Level

biological source

bovine

form

lyophilized powder

potency

15 - 200 μg/mL EC50

quality

endotoxin tested

packaging

pkg of 5 mg

application(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mg

color

brown

solubility

0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange

storage temp.

2-8°C

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General description

Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.

Application

Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells. It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.

Biochem/physiol Actions

Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity and promotes the growth of several types of mammalian cells. It favors the proliferation of keratocytes culture and bovine knee chondrocytes. BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.

Physical form

Lyophilized from sodium phosphate buffer

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Yung-Chih Kuo et al.
Biotechnology progress, 21(6), 1708-1715 (2005-12-03)
In vitro cultivation of primary bovine knee chondrocytes (BKCs), using bovine pituitary extract (BPE) and porous scaffolds composed of polyglycolide (PGA) and 85/15 poly(lactide-co-glycolide) (PLGA), was investigated. Here, BPE was prepared from fresh bovine pituitaries, and cylindrical PGA/PLGA scaffolds with...
Simon W Hayward et al.
Methods in molecular biology (Clifton, N.J.), 218, 17-33 (2003-03-06)
The ability to rescue viable prostate precursor tissue from Rb-/-fetal mice has allowed for the generation of Rb-/-prostate tissue and Rb-/-prostate epithelial cell lines. Herein, we provide a protocol for the rescue of urogenital precursor tissue from mouse embryos harboring...
Zhong-Zhong Xu et al.
International journal of ophthalmology, 6(6), 758-765 (2014-01-07)
To investigate the effects of bovine pituitary extract on the proliferation of keratocytes and maintaining the keratocyte phenotype in vitro. Single keratocytes were isolated by enzyme digestion for in vitro culture. Three groups were designed according to the different culture...
T M Brushart et al.
Experimental neurology, 247, 272-281 (2013-05-28)
Myelinating Schwann cells express distinct sensory and motor phenotypes as defined by their differing patterns of growth factor production (Hoke et al., 2006). The heterogeneous growth factor requirements of sensory and motor neurons, however, suggest that Schwann cell phenotype might...
J C Willey et al.
Journal of cellular physiology, 124(2), 207-212 (1985-08-01)
The mechanisms of action of extracellular mitogens for normal human bronchial epithelial cells (NHBE) were investigated by observing their effects on selected biochemical pathways when the cells were incubated in serum-free media. We find that (a) epidermal growth factor (EGF)...

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