P1526

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, solid

• Synonym(s): OPD
• Linear Formula: C₆H₄(NH₂)₂·2HCl
• CAS Number: 615-28-1
• Molecular Weight: 181.06
• Beilstein/REAXYS Number: 3912045
• EC Number: 210-418-7
• MDL number: MFCD00012966
• UNSPSC Code: 12352204
• PubChem Substance ID: 24898213
• NACRES: NA.83

Properties

• Product Name: o-Phenylenediamine dihydrochloride, peroxidase substrate
• Quality Level: 300
• form: solid
• color: white to off-white
• mp: 258 °C (dec.) (lit.)
• storage temp.: −20°C
• SMILES string: Cl[H].Cl[H].Nc1ccccc1N
• InChI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChI key: RIIWUGSYXOBDMC-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.[1][2][3][4]

Caution

May darken in storage.

Safety Information

• pictograms: GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301,H312 + H332,H317,H319,H341,H351,H410
• pcodes: P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable