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P1530

Sigma-Aldrich

Polyinosinic–polycytidylic acid sodium salt

TLR ligand tested

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Synonym(s):
Poly (I:C), Poly(I) • Poly(C)
CAS Number:
MDL number:
NACRES:
NA.51

Quality Level

Assay

≥99% (less than 1% free nucleotides, TLC)

form

lyophilized powder

storage condition

desiccated

solubility

H2O: 10 mg/mL

storage temp.

−20°C

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This Item
P0913P9582I3036
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

-

solubility

H2O: 10 mg/mL

solubility

H2O: 10 mg/mL

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

-

storage condition

desiccated

storage condition

desiccated

storage condition

desiccated

storage condition

-

Application

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Other Notes

Double-stranded homopolymer.

Preparation Note

The product requires ionic strength to maintain the double-strand structure. To prevent denaturation, reconstitute in solutions with physiological salt concentrations (e.g., saline solution). Reconstitution may require heating (50 °C) and cooling to achieve re-annealing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Yipeng Ren et al.
Fish & shellfish immunology, 70, 720-730 (2017-09-30)
Toll-like receptors, the best known pattern recognition receptors, play important roles in recognizing non-self molecules and binding pathogen-associated molecular patterns in the innate immune system. In the present research, the cDNA and protein characterization of the TLR signalling pathway genes
Pei-Hui Wang et al.
Nucleic acids research, 46(8), 4054-4071 (2018-03-17)
STING is a core adaptor in innate nucleic acid sensing in mammalian cells, on which different sensing pathways converge to induce type I interferon (IFN) production. Particularly, STING is activated by 2'3'-cGAMP, a cyclic dinucleotide containing mixed phosphodiester linkages and
Anutosh Paria et al.
Developmental and comparative immunology, 78, 71-82 (2017-09-19)
MDA5 is the pivotal member of the retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) and is reported to play a crucial role in type I IFN-mediated responses against pathogen-associated molecular patterns (PAMPs), especially nucleic acids. In this study, we have
Svenja Fritzlar et al.
mBio, 10(3) (2019-06-20)
The integrated stress response (ISR) is a cellular response system activated upon different types of stresses, including viral infection, to restore cellular homeostasis. However, many viruses manipulate this response for their own advantage. In this study, we investigated the association
Ana Miar et al.
Cancer research, 80(23), 5245-5256 (2020-10-30)
Hypoxia is a common phenomenon in solid tumors and is strongly linked to hallmarks of cancer. Recent evidence has shown that hypoxia promotes local immune suppression. Type I IFN supports cytotoxic T lymphocytes by stimulating the maturation of dendritic cells

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