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P1598

Sigma-Aldrich

PCR 20 bp Low Ladder

for electrophoresis of PCR fragments

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Synonym(s):
20 bp Low Ladder, Marker for DNA gel electrophoresis
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid

usage

80 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

2-8°C

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P1598

PCR 20 bp Low Ladder

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D3687

DirectLoad PCR 100 bp Low Ladder

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P1473

PCR 100 bp Low Ladder

vibrant-m

P9577

PCR 50 - 2,000 bp Marker

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

liquid

form

liquid

form

liquid

form

liquid

usage

80 uses

usage

75 uses

usage

75 uses

usage

sufficient for 50 uses

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

General description

Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.

Components

Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Articles

We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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