Skip to Content
MilliporeSigma

P1709

Peroxidase, Maleimide Activated from horseradish

lyophilized powder, >200 units/mg protein

Synonym(s):

Maleimide activated HRP

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

2 MG

$261.00

3 X 2 MG

$495.00

$261.00

Check Cart for Availability

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00284131
Specific activity:
>200 units/mg protein
Biological source:
horseradish

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

horseradish

form

lyophilized powder

specific activity

>200 units/mg protein

composition

Protein, ~50% E1%/403

extent of labeling

1-3 mol maleimide per mol protein

storage temp.

−20°C

Quality Level

200

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
11428861001P208810108090001
Peroxidase, Maleimide Activated from horseradish lyophilized powder, >200 units/mg protein

Sigma-Aldrich

P1709

Peroxidase, Maleimide Activated from horseradish

Peroxidase (POD), activated from horseradish

Roche

11428861001

Peroxidase (POD), activated

Peroxidase from horseradish Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)

Sigma-Aldrich

P2088

Peroxidase from horseradish

Peroxidase (POD) grade I, from horseradish

Roche

10108090001

Peroxidase (POD)

biological source

horseradish

biological source

horseradish

biological source

horseradish

biological source

horseradish

specific activity

>200 units/mg protein

specific activity

800 units/mg protein (at 25 °C with ABTS and H2O2 as substrate (pH 5.0).)

specific activity

200-300 units/mg solid (using pyrogallol)

specific activity

≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.), 250 U/mg

form

lyophilized powder

form

lyophilized

form

essentially salt-free, lyophilized powder

form

lyophilized (salt-free)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

300

Quality Level

100

composition

Protein, ~50% E1%/403

composition

-

composition

-

composition

-

General description

Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms.[1]

Application

For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent.[2]
Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. [3] This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase. [4]
To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification. [5]

Biochem/physiol Actions

Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc.[1]

Physical form

Lyophilized powder containing trehalose and sodium citrate

Other Notes

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20°C.
Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCR4408
SLCR4408
SLCN0574
SLCL2484
SLCG9173

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Biochemical Methods (1996)
Subhash Dhawan
Peptides, 23(12), 2091-2098 (2003-01-22)
Immunoconjugates are widely used for indirect detection of analytes (such as antibodies or antigens) in a variety of immunoassays. However, the availability of functional groups such as primary amines or free sulfhydryls in an immunoglobulin molecule is the limiting factor
S Matuda et al.
In vivo (Athens, Greece), 1(4), 221-227 (1987-07-01)
IgG-maleimide peroxidase, Fab'-maleimide peroxidase, polymer and monomer types of Fab'-periodate peroxidase were prepared from an antibody against rat lipoamide dehydrogenase, a component of the pyruvate dehydrogenase complex which is located in mitochondria. They were examined for immunohistochemical staining of the
S Hashida et al.
Journal of applied biochemistry, 6(1-2), 56-63 (1984-02-01)
Nine different maleimide compounds were evaluated for the conjugation of Fab' to horseradish peroxidase through thiol groups in the hinge. The compounds evaluated were succinimidyl maleimidoacetate (I), succinimidyl 4-maleimidobutyrate (II), succinimidyl 6-maleimidohexanoate (III), succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (IV), succinimidyl m-maleimidobenzoate (V), succinimidyl
Kitagawa, T., et al.
Chemical & Pharmaceutical Bulletin, 29(4), 1131-1131 (1981)
View All Related Papers

Protocols

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Related Content

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service