MilliporeSigma
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P1995

Sigma-Aldrich

Anti-Podoplanin (HG-19) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~38 kDa

species reactivity

rat

technique(s)

indirect immunofluorescence: 10-20 μg/mL using rat kidney frozen sections
western blot (chemiluminescent): 2.5-5 μg/mL using whole extract of rat kidney glomeruli and rat lung

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Pdpn(54320)

General description

Podoplanin is present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is also named E11 antigen and has been described to be present in normal, inflammatory, and neoplastic lymphatic capillary endothelium. Podoplanin is expressed selectively by dermal microvascular lymphatic endothelial cells but not by blood vascular endothelial cells. It is composed of heavily glycosylated extracellular domain and a short intracytoplasmic tail.
Podoplanin is part of the type-1 transmembrane sialomucin-like glycoprotein family. The gene encoding this 43kDa protein is localized on rat chromosome 5.

Immunogen

synthetic peptide of rat podoplanin (amino acids 111-129) with N-terminal cysteine, conjugated to KLH. The sequence in mouse differs by one residue.

Application

Anti-Podoplanin (HG-19) antibody produced in rabbit has been used in immunostaining, immunoblotting and immunofluorescence staining.

Packaging

200 μL in poly bottle

Biochem/physiol Actions

Podoplanin is a marker of lymphatic endothelial cells. It may have a role in tumor cell invasion and migration.
Podoplanin expression is regulated by the lymphatic-specific homeobox gene Prox-1. Podoplanin appears to play an important role in the maintenance of podocyte foot processes, and hence, glomerular permeability. It has a critical regulatory role in the formation of the lymphatic vasculature. Podoplanin does not seem to be upregulated in tumor angiogenesis. It is detectable in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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