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P2192

Sigma-Aldrich

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

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NACRES:
NA.52

Quality Level

form

liquid

packaging

vial of 1.5 mL

color

colorless

application(s)

agriculture

foreign activity

DNase, RNase, none detected

storage temp.

−20°C

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P2317L6669S5193
Sigma-Aldrich

Sigma-Aldrich

P2192

10X PCR Buffer

color

colorless

color

colorless

color

colorless

color

colorless

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

foreign activity

DNase, RNase, none detected

foreign activity

-

foreign activity

-

foreign activity

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

Application

10X PCR Buffer has been used as a component of the PCR mixture:
  • for the amplification of viral DNA
  • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
  • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

Features and Benefits

  • Standalone buffer
  • Compatible with JumpStart Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

Components

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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705578-5MG-PW

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1000309185

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Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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