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P2318

Sigma-Aldrich

Anti-Pseudomonas Exotoxin A antibody produced in rabbit

whole antiserum

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Synonym(s):
Anti-ETA
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

(Pseudomonas aeruginosa)

technique(s)

dot blot: 1:20,000
indirect ELISA: 1:250,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Pseudomonas aeruginosa PAO1 ... toxA(877850)

General description

Reacts against Pseudomonas exotoxin A, but shows no reaction against Staphylococcal enterotoxin A, Cholera toxin, and Staphylococcal enterotoxin B. The product has not been tested for neutralization potency against active Pseudomonas exotoxin A.

Immunogen

exotoxin A from Pseudomonas aeruginosa.

Application

Anti-Pseudomonas Exotoxin A antibody has been used in in vitro serum stability of fusion toxins and binding of targeted toxins.
Anti-Pseudomonas exotoxin A antibody produced in rabbit has been used:
  • in the in vitro analysis of the stability of fusion toxins in serum
  • to construct an epidermal growth factor receptor (EGFR)-targeted toxin to study its toxicity on Swiss mouse embryo NIH-3T3 cells transfected with human EGFR
  • in the determination of the in vivo half-life of immunotoxins in serum

Biochem/physiol Actions

Pseudomonas exotoxin A is secreted by Pseudomonas aeruginosa and has potent cytotoxic effects. Exotoxin A enters the cells by receptor-mediated endocytosis, translocates to the cytoplasm and inhibits protein synthesis by ADP-ribosylating factor 2. Chimeric exotoxin A has applications in gene treatment of various diseases and probable treatment for cancer.

Physical form

This product is supplied as a liquid containing 15 mM sodium azide as preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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