P246

Sigma-Aldrich

Monoclonal Anti-PSD95 antibody produced in mouse

clone 7E3-1B8, purified immunoglobulin, buffered aqueous solution

MDL number:

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7E3-1B8, monoclonal

form

buffered aqueous solution

mol wt

antigen 95 kDa

species reactivity

rat

application(s)

immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:200
western blot: 1:2,000

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Dlg4(29495)

General description

DLG4 (discs large MAGUK scaffold protein 4) gene codes for the postsynaptic density-95 (PSD95) protein. Postsynaptic density 95 (PSD-95), a synaptic scaffolding protein is also a membrane-associated guanylate kinase (MAGUK) family protein. It has three PDZ domains on the N terminus followed by a Src homology 3 (SH3) domain and a guanylate kinase (GK) domain. This gene is located on human chromosome 17p13.1.

Specificity

Reacts with both the recombinant and native rat PSD95. By immunofluorescence with rat hippocampal cells, a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with its proposed role in receptor clustering is observed. Fixation with cold methanol is recommended.

Immunogen

recombinant rat PSD95 (post synaptic density 95 kDa).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Postsynaptic density 95 (PSD-95) plays a major role in bidirectional synaptic plasticity, an important process, essential for learning and memory. It exhibits increased dynamics upon induction of plasticity.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Lindsey I Sinclair et al.
Neuropathology and applied neurobiology, 41(4), 533-543 (2015-01-07)
Cerebral ischaemia is the defining pathophysiological abnormality in most forms of vascular dementia (VAD), but the pathogenesis of the dementia remains poorly understood. In Alzheimer's disease (AD), there is early loss of synaptic proteins, but these have been little studied...
Capping of the N-terminus of PSD-95 by calmodulin triggers its postsynaptic release
Yonghong Zhang
The Embo Journal (2014)
Malwina Lisek et al.
Frontiers in cellular neuroscience, 11, 181-181 (2017-07-14)
Ketamine causes psychotic episodes and is often used as pharmacological model of psychotic-like behavior in animals. There is increasing evidence that molecular mechanism of its action is more complicated than just N-methyl-D-aspartic acid (NMDA) receptor antagonism and involves interaction with...
Postsynaptic density 95 (PSD-95) serine 561 phosphorylation regulates a conformational switch and bidirectional dendritic spine structural plasticity
Wu Q, et al.
The Journal of biological chemistry, jbc-M117 (2017)
Human postsynaptic density-95 (PSD95): location of the gene (DLG4) and possible function in nonneural as well as in neural tissues
Stathakis D G, et al.
Genomics, 44(1), 71-82 (1997)

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