P2482

Sigma-Aldrich

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse

clone PKB-175, ascites fluid

Synonym(s):
Anti-PKBα, Anti-Akt1
MDL number:
NACRES:
NA.44

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PKB-175, monoclonal

mol wt

antigen 56 kDa

contains

15 mM sodium azide

species reactivity

chicken, rat, bovine, mouse, human

application(s)

indirect ELISA: suitable
microarray: suitable
western blot: 1:5,000 using human MCF-7 cell extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)

General description

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) (mouse IgG1 isotype) is derived from the PKB-175 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. PKB/Akt is composed of an N-terminal pleckstrin-homology (PH) domain, followed by a catalytic kinase domain and a short C-terminal regulatory domain. This cytoplasmic protein is made up of 480 amino acids. AKT1 is mapped to human chromosome 14q32.
Protein Kinase B (also known as Akt, or RAC-PK) is a protein that belongs to serine/threonine kinases.

Specificity

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) recognizes PKB isoform α.

Immunogen

Synthetic peptide corresponding to amino acids 461-477 of human PKBα/Akt1, conjugated to KLH. The sequence is identical in mouse, rat, and bovine PKBα/Akt1, highly conserved in PKBβ/Akt2, and diverges in PKBγ.

Application

Monoclonal Anti-Protein Kinase Bα antibody produced in mouse has been used in:
  • western blot
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Protein kinase Bα (PKBα/Akt1) plays a crucial role as a suppressor of apoptotic cell death in different cell types, induced by a variety of stimuli including growth factor withdrawal, loss of cell adhesion and DNA damage. It can protect cerebellar neurons from apoptosis induced by insulin-like growth factor 1 (IGF-1) withdrawal. Akt is the cellular homologue of the viral oncogene v-akt of the AKT-8 acute transforming retrovirus found in rodent T cell lymphoma. It is considered to play an important role in the control of cell cycle, cell proliferation and differentiation . AKT plays a key role in angiogenesis.

Physical form

Monoclonal Anti-Protein Kinase Bα (PKBα/Akt1) is supplied as ascites fluid containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2-8C for upto one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

nwg

Certificate of Analysis
Certificate of Origin
Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells
Garcia RA, et al.
Molecular Cancer, 12(1), 44-44 (2013)
AKT1 (v-akt murine thymoma viral oncogene homolog 1)
Etro D, et al.
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2010)
null
Jinling Wu et al.
Development (Cambridge, England), 133(18), 3651-3660 (2006-08-18)
GIPC is a PDZ-domain-containing protein identified in vertebrate and invertebrate organisms through its interaction with a variety of binding partners including many membrane proteins. Despite the multiple reports identifying GIPC, its endogenous function and the physiological significance of these interactions...
Kedar S Vaidya et al.
The Journal of biological chemistry, 283(42), 28354-28360 (2008-07-31)
That metastatic tumor cells grow in selective non-native environments suggests an ability to differentially respond to local microenvironments. BRMS1, like other metastasis suppressors, halts ectopic growth (metastasis) without blocking orthotopic tumor formation. BRMS1-expressing tumor cells reach secondary sites but do...
Articles
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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