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P2645

Sigma-Aldrich

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

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Synonym(s):
PKA
Enzyme Commission number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.32

form

lyophilized (white powder to sticky mass to hard pellet)

Quality Level

specific activity

≥9 units/μg protein (cyclic-AMP is not required for this activity)

mol wt

40,862 Da

storage temp.

−20°C

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This Item
P5511C8482P6998
Sigma-Aldrich

P5511

Protein Kinase A from bovine heart

form

lyophilized (white powder to sticky mass to hard pellet)

form

lyophilized powder

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

mol wt

40,862 Da

mol wt

-

mol wt

43.5 kDa

mol wt

protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

General description

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.

Application

Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
  • to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
  • in in Vitro PKA pPhosphorylation assay
  • in Vitro affinity binding assays
  • to study effects of PKA on inspiratory drive currents in functionally active motorneurons

Biochem/physiol Actions

Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.

Packaging

Package size based on phosphorylating units

Unit Definition

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP.

Physical form

Lyophilized powder with sucrose and phosphate buffer salts as stabilizer.

Preparation Note

Prepared from protein kinase A (P 5511)

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Petr G Vikhorev et al.
Cardiovascular research (2020-11-03)
Dilated cardiomyopathy (DCM) is associated with mutations in many genes encoding sarcomere proteins. Truncating mutations in the titin gene TTN are the most frequent. Proteomic and functional characterisations are required to elucidate the origin of the disease and the pathogenic
E Wischmeyer et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5819-5823 (1996-06-11)
Strongly rectifying IRK-type inwardly rectifying K+ channels are involved in the control of neuronal excitability in the mammalian brain. Whole-cell patch-clamp experiments show that cloned rat IRK1 (Kir 2.1) channels, when heterologously expressed in mammalian COS-7 cells, are inhibited following
J A Byun et al.
Science advances, 6(25), eabb1250-eabb1250 (2020-07-01)
The functional response of a signaling system to an allosteric stimulus often depends on subcellular conditions, a phenomenon known as pluripotent allostery. For example, a single allosteric modulator, Rp-cAMPS, of the prototypical protein kinase A (PKA) switches from antagonist to
Christopher M Bocchiaro et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(4), 1099-1103 (2003-02-25)
Plasticity underlying adaptive, long-term changes in breathing behavior is hypothesized to be attributable to the modulation of respiratory motoneurons by intracellular second-messenger cascades. In quiescent preparations, protein kinases, including cAMP-dependent protein kinase A (PKA), potentiate glutamatergic inputs. However, the dynamic
Cyclic nucleotides in the nervous system
Basic Neurochemistry, 423-441 (2012)

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