P2645

Sigma-Aldrich

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

Synonym(s):
PKA
Enzyme Commission number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.32

Quality Level

form

lyophilized (white powder to sticky mass to hard pellet)

specific activity

≥9 units/μg protein (cyclic-AMP is not required for this activity)

mol wt

40,862 Da

storage temp.

−20°C

General description

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.

Application

Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
  • to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
  • in in Vitro PKA pPhosphorylation assay
  • in Vitro affinity binding assays
  • to study effects of PKA on inspiratory drive currents in functionally active motorneurons

Biochem/physiol Actions

Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.

Packaging

Package size based on phosphorylating units

Unit Definition

One unit will transfer 1.0 picomole of phosphate from γ-32P-ATP to hydrolysed and partially dephosphorylated Casein per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing ≤ 1% protein with sucrose and phosphate buffer salts as stabilizer

Preparation Note

Prepared from protein kinase A (P 5511)

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Ken Tougane et al.
Plant physiology, 152(3), 1529-1543 (2010-01-26)
Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the...
F T Hartl et al.
The Journal of biological chemistry, 258(6), 3950-3955 (1983-03-25)
The physical and chemical properties of purified catalytic and regulatory subunits of type II cAMP-dependent protein kinase from bovine brain, skeletal muscle, and cardiac muscle were compared. The catalytic subunits from all three sources were identical with respect to molecular...
Cyclic nucleotides in the nervous system
Basic Neurochemistry, 423-441 (2012)
E Wischmeyer et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5819-5823 (1996-06-11)
Strongly rectifying IRK-type inwardly rectifying K+ channels are involved in the control of neuronal excitability in the mammalian brain. Whole-cell patch-clamp experiments show that cloned rat IRK1 (Kir 2.1) channels, when heterologously expressed in mammalian COS-7 cells, are inhibited following...
Protein kinase A activity and Hedgehog signaling pathway
Vitamins & Hormones, 88, 273-291 (2012)

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