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  • P2645
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P2645

Sigma-Aldrich

Protein Kinase A Catalytic Subunit from bovine heart

≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)

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Synonym(s):
PKA
Enzyme Commission number:
2.7.11.11 (BRENDA, IUBMB)
MDL number:
MFCD00132114
eCl@ss:
32160410
NACRES:
NA.32

form

lyophilized (white powder to sticky mass to hard pellet)

Quality Level

200

specific activity

≥9 units/μg protein (cyclic-AMP is not required for this activity)

mol wt

40,862 Da

storage temp.

−20°C

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P2645

Protein Kinase A Catalytic Subunit from bovine heart

Protein kinase A inhibitor fragment 5-24 amide trifluoroacetate salt ≥95% (HPLC), lyophilized powder

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PI3 kinase [p110a(H1047R)/p85a] Active human recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

Sigma-Aldrich

SRP0235

PI3 kinase [p110a(H1047R)/p85a] Active human

form

lyophilized (white powder to sticky mass to hard pellet)

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

specific activity

≥9 units/μg protein (cyclic-AMP is not required for this activity)

specific activity

-

specific activity

-

specific activity

≥1770 pmol/min-μg

mol wt

40,862 Da

mol wt

calculated mol wt 2219

mol wt

-

mol wt

129 kDa (p110α), 88 kDa (p85α)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

General description

Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.

Application

Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
  • to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
  • in in Vitro PKA pPhosphorylation assay
  • in Vitro affinity binding assays
  • to study effects of PKA on inspiratory drive currents in functionally active motorneurons

Biochem/physiol Actions

Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.

Packaging

Package size based on phosphorylating units

Unit Definition

One unit will transfer 1.0 picomole of phosphate from γ-32P-ATP to hydrolysed and partially dephosphorylated Casein per min at pH 6.5 at 30 °C.

Physical form

Lyophilized powder containing ≤ 1% protein with sucrose and phosphate buffer salts as stabilizer

Preparation Note

Prepared from protein kinase A (P 5511)

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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