P2783

Phosphotransacetylase from Bacillus stearothermophilus

Name: Phosphotransacetylase from <I>Bacillus stearothermophilus</I>
Brand: SIGMA
Price: 760 USD

lyophilized powder, ≥3,000 units/mg protein (biuret)

Synonym(s):

Acetyl-CoA:orthophosphate acetyltransferase, Phosphate acetyltransferase

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5000 UNITS

$760.00

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About This Item

CAS Number:

9029-91-8

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-855-2

MDL number:

MFCD00131907

EC Number:

2.3.1.8 (BRENDA, IUBMB)

Key Documents

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Product Name

Phosphotransacetylase from Bacillus stearothermophilus, lyophilized powder, ≥3,000 units/mg protein (biuret)

form

lyophilized powder

specific activity

≥3,000 units/mg protein (biuret)

composition

Protein, ≥20%

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

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This Item	C4899	SAB4504108	SAB1306205
Sigma-Aldrich P2783 Phosphotransacetylase from Bacillus stearothermophilus	Sigma-Aldrich C4899 Carnitine Acetyltransferase from pigeon breast muscle	Sigma-Aldrich SAB4504108 Anti-phospho-TIP60 (pSer⁸⁶) antibody produced in rabbit	Sigma-Aldrich SAB1306205 ANTI-HMOF/MYST1(C-TERMINAL) antibody produced in rabbit
specific activity ≥3,000 units/mg protein (biuret)	specific activity ≥50 units/mg protein	specific activity -	specific activity -
form lyophilized powder	form ammonium sulfate suspension	form buffered aqueous solution	form buffered aqueous solution
shipped in wet ice	shipped in -	shipped in wet ice	shipped in wet ice
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
composition Protein, ≥20%	composition -	composition -	composition -

Application

Phosphotransacetylase, from Bacillus stearothermophilus, is used to convert CoA to acetyl CoA. Phosphotransacetylase (pta) is used to study transport systems for acetate[1]. It is used to study metabolic pathways in various bacterium[2].

Biochem/physiol Actions

Phosphotransacetylase converts CoA to acetyl CoA.

Other Notes

One unit will convert 1.0 μmole of CoA to acetyl CoA per min at pH 7.5 at 30 °C using acetyl phosphate as substrate.

Physical form

Lyophilized powder containing potassium phosphate

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000454533

0000310597

0000272087

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Metabolic Pathways in Methanococcus jannaschii and Other Methanogenic Bacteria.

G D Sprott et al.

Applied and environmental microbiology, 59(4), 1092-1098 (1993-04-01)

Eleven strains of methanogenic bacteria were divided into two groups on the basis of the directionality (oxidative or reductive) of their citric acid pathways. These pathways were readily identified for most methanogens from the patterns of carbon atom labeling in

Mutants of Bacillus stearothermophilus defective in the uptake and metabolism of acetate

Philip R. Mallinder and Anne Moir

Microbiology, 137, 779-785 (1991)

Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant.

Shital A Tripathi et al.

Applied and environmental microbiology, 76(19), 6591-6599 (2010-08-10)

We report development of a genetic system for making targeted gene knockouts in Clostridium thermocellum, a thermophilic anaerobic bacterium that rapidly solubilizes cellulose. A toxic uracil analog, 5-fluoroorotic acid (5-FOA), was used to select for deletion of the pyrF gene.

The cytoplasmic cyclophilin from Azotobacter vinelandii interacts with phosphate acetyltransferase isoforms enhancing their in vitro activity.

Maria Dimou et al.

Molecular biology reports, 39(4), 4135-4143 (2011-07-21)

Cyclophilins belong to the peptidyl-prolyl cis/trans isomerase family of enzymes (EC 5.2.1.8), which accelerate protein folding by catalysing the cis/trans isomerisation of proline imidic peptide bonds. In the present study, by a combination of bioinformatics methods, we identify phosphate acetyltransferase

Mechanism of thiol-supported arsenate reduction mediated by phosphorolytic-arsenolytic enzymes: II. Enzymatic formation of arsenylated products susceptible for reduction to arsenite by thiols.

Zoltán Gregus et al.

Toxicological sciences : an official journal of the Society of Toxicology, 110(2), 282-292 (2009-05-30)

Enzymes catalyzing the phosphorolytic cleavage of their substrates can reduce arsenate (AsV) to the more toxic arsenite (AsIII) via the arsenolytic substrate cleavage in presence of a reductant, as glutathione or dithiotreitol (DTT). We have shown this for purine nucleoside

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Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

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