P2860

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03457573
• Conjugate: unconjugated
• Clone: B92, monoclonal
• Application: ARR, ICC, IHC (f), IP, RIA, WB
• Citations: 37

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: B92, monoclonal
• form: buffered aqueous solution
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, radioimmunoassay: suitable using cells, western blot: 2-4 μg/mL using whole extract of cultured HeLa cells
• isotype: IgG1
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TAP1(6890)

Description

General description

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

Immunogen

lysate of mouse bone marrow-derived stromal cell line.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

Monoclonal Anti-PSF antibody produced in mouse has been used in:

• immunofluorescence
• immunohistochemistry
• immunoprecipitation

Biochem/physiol Actions

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable