No, this ready mix has typical cycling parameters for 0.2–2 kb fragments
P2893
JumpStart™ Taq ReadyMix™
Complete optimized reagent for hot-start PCR at 2X concentration
Synonym(s):
hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR
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| Size/SKU | Availability | Price |
|---|---|---|
100 reactions | Estimated to ship TODAYfromSAINT LOUIS | $206.00 |
400 reactions | Estimated to ship TODAYfromSAINT LOUIS | $676.00 |
About This Item
Concentration:
2.5 units/reaction (50 μL reaction volume)
Quality Segment
form
liquid
usage
sufficient for 100 reactions, sufficient for 400 reactions
feature
Multiplex PCR, dNTPs included, hotstart
concentration
2.5 units/reaction (50 μL reaction volume)
technique(s)
PCR: suitable using : suitable, WGA
color
colorless
suitability
suitable for (quantitative PCR)
application(s)
agriculture
shipped in
wet ice
storage temp.
−20°C
General description
JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains JumpStart™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart™ Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
Application
JumpStart™ Taq ReadyMix™ has been used in quantitative polymerase chain reaction (PCR).[1] It has also been used to identify fibroblast cell surface adenosine receptor (AR) types under hypoxia. It is also suitable:
- For PCR amplifications that require reduced non-specific amplification
- For multiplex PCR
- For reduction of primer dimers
Features and Benefits
- The master mix allows consistency from one reaction to the next
- Reduced preparation time and reduced risk of contamination from multiple pipetting steps
- Designed to minimize non-specific amplification and contamination
- Increased specificity and target yield
- Reduced primer dimers
- Reduced set-up time as compared to manual or wax Hot Start methods
- Allows for room temperature set-up
- Ideal for high throughput, quantitative PCR applications
Packaging
Default reaction volume is 50 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
Other Notes
JumpStart Taq ReadyMix is a prepared solution combining the performance benefits of hot start PCR with the convenience of a ReadyMix. The mix includes JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. JumpStart Taq Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq polymerase does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The hot start mechanism allows for room temperature set up, making it ideal for high throughput applications. To prepare a 50 μL reaction, simply add 25 μL of ReadyMix to template, primers and water for a final reaction volume of 50 μL.
To prepare a 50 μL reaction, simply add 25 μL of ReadyMix™ to template, primers and water for a final reaction volume of 50 μL.
This product has been validated in quantitative PCR, but may require supplementation with magnesium chloride solution, 25 mM, Catalog Number M8787, a suitable fluorescent probe, and, if desired, an internal reference dye, Catalog Number R4526. View more detailed information on JumpStart Taq ReadyMix products at www.sigma-aldrich.com/hotstart.
This product has been validated in quantitative PCR, but may require supplementation with magnesium chloride solution, 25 mM, Catalog Number M8787, a suitable fluorescent probe, and, if desired, an internal reference dye, Catalog Number R4526. View more detailed information on JumpStart Taq ReadyMix products at www.sigma-aldrich.com/hotstart.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description Complete optimized reagent for hot-start PCR at 2X concentration | description for quantitative PCR, without MgCl2 in buffer | description for High-throughput PCR of complex templates | description for PCR |
| technique(s) PCR: suitable | technique(s) RT-qPCR: suitable | technique(s) PCR: suitable | technique(s) PCR: suitable |
| application(s) agriculture | application(s) agriculture | application(s) agriculture | application(s) agriculture |
| suitability suitable for (quantitative PCR) | suitability suitable for PCR | suitability - | suitability - |
| form liquid | form liquid | form liquid | form liquid |
| concentration 2.5 units/reaction (50 μL reaction volume) | concentration 1.25 units/reaction (50 μL reaction volume) | concentration 0.75 units/reaction (50 μL reaction volume) | concentration 2.5 units/reaction (50 μL reaction volume) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can the Jumpstart ready mix be used for long range PCR with amplicon size of approximately 20kb
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can PCR products made with Product P2893, JumpStart™ Taq ReadyMix™, be used for TA cloning?
1 answer-
Yes, this enzyme does not have 3'-5' exonuclease activity, so there will be 3'A overhangs available for this type of cloning approach.
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Can Product P2893, JumpStart™ Taq ReadyMix™, be used with Bio-Rad real-time PCR machines?
1 answer-
Yes. Bio-Rad's real-time instruments typically require fluorescein as an internal reference dye. We have fluorescein as product F6377 (Fluorescein, sodium salt).
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Can Product P2893, JumpStart™ Taq ReadyMix™, be used in real-time PCR?
1 answer-
Yes. The mixture does not contain any internal reference dye, though. D7440 comes with a vial of our internal reference dye, R4526, which can also be purchased separately.
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What is the fidelity of Taq DNA Polymerase?
1 answer-
The error rates for polymerases are more accurately presented in terms of ranges since the actual fidelity is condition dependent. The error rate of standard Taq, as reported in the literature, is in a range of 1 in 1.1 × 104 (Tindall, K.R., and Kunkel, T.A., Biochemistry, 27, 6008-6013 (1988)) to 1 in 2.0 × 105 (Lundberg, K.S., Shoemaker, D.D., Adams, M.W., Short, J.M., Sorge, J.A.and Mathur, E.J., Gene, 108(1), 1-6 (1991)). These values would apply to this product.
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