P2983

Millipore

ANTI-FLAG® High Sensitivity, M2 coated 96-well plates

96-well, clear, polystyrene, flat bottom plate

Synonym(s):
Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG M2 antibody produced in mouse
NACRES:
NA.32

Quality Level

antibody product type

primary antibodies

material

polystyrene

clone

monoclonal

shelf life

Unopened plates are stable for 2 years. Once opened they are stable for 2 weeks.

application(s)

ELISA: suitable

isotype

IgG1

capacity

100-300 ng/well

storage temp.

2-8°C

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General description

The Anti-FLAG® HS, M2 Coated Plates are designed to ensure a higher binding capacity than other plates. This is due to the orientation of the Anti-FLAG® M2 mouse monoclonal IgG1 antibody on the plates. The antibody is covalently linked to the surface of a microtiter plate via the Fc portion of the antibody.

Application

ANTI-FLAG® High Sensitivity, M2 coated 96-well plates is used for Enzyme-linked immunosorbent assays. A convenient, ready to use, platform for the capture and detection of FLAG® fusion proteins. The ANTI-FLAG® M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigma′s GMP facility, ANTI-FLAG®P2983 high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.

Browse additional application references in our
FLAG® Literature portal.

Storage and Stability

Once plates are opened, they should be stored with desiccant at 2- 8 °C and used within two weeks.

Other Notes

Components:
The plate is supplied as a 96-well microtiter plate with clear sides and bottom.
Coating:
ANTI-FLAG® M2 mouse monoclonal antibody, IgG1, is coated at a reaction volume of 200 ml/well.
Blocking:
The wells are pre-blocked for convenience at 275 to 300 ml/well with a complex solution containing bovine serum albumin.
Specificity:
The plates are specific for the FLAG epitope regardless of its placement in the fusion protein: amino-terminal, Met-amino terminal, carboxy terminal or internal. Binding of the epitope is not Ca2+ dependent.
Sensitivity:
Detection of 1 ng/well of a control fusion protein was observed in an ELISA format with p-Nitrophenyl Phosphate (pNPP) as a substrate.
Capacity:
Capture of 100 to 300 ng/well of a FLAG fusion protein has been demonstrated.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
F-127 is a registered trademark of BASF SE
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Shu Kachi et al.
Human gene therapy, 20(1), 31-39 (2009-01-01)
Equine infectious anemia virus (EIAV) is a nonprimate lentivirus that does not cause human disease. Subretinal injection into mice of a recombinant EIAV lentiviral vector in which lacZ is driven by a CMV promoter (EIAV CMV LacZ) resulted in rapid...
FLAG 96-well Immunoprecipitation System for High-Throughput Protein-Protein Interaction Studies
Uder, S., et al.
Molecular Biology, 4 null
Molecular dissection of the interaction between the AMPA receptor and cornichon homolog-3.
Shanks NF, Cais O, Maruo T, et al.
The Journal of Neuroscience, 34(36), 12104-12120 (2014)
Rahul Bhattacharjee et al.
Molecular biology of the cell, 31(9), 917-929 (2020-02-27)
In many organisms, positive and negative signals cooperate to position the division site for cytokinesis. In the rod-shaped fission yeast Schizosaccharomyces pombe, symmetric division is achieved through anillin/Mid1-dependent positive cues released from the central nucleus and negative signals from the...
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