ANTI-FLAG® High Sensitivity, M2 coated 96-well plates

96-well, clear, polystyrene, flat bottom plate

Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG M2 antibody produced in mouse

Quality Level

antibody product type

primary antibodies





shelf life

Unopened plates are stable for 2 years. Once opened they are stable for 2 weeks.


ELISA: suitable




100-300 ng/well

storage temp.


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General description

The Anti-FLAG® HS, M2 Coated Plates are designed to ensure a higher binding capacity than other plates. This is due to the orientation of the Anti-FLAG® M2 mouse monoclonal IgG1 antibody on the plates. The antibody is covalently linked to the surface of a microtiter plate via the Fc portion of the antibody.


ANTI-FLAG® High Sensitivity, M2 coated 96-well plates is used for Enzyme-linked immunosorbent assays. A convenient, ready to use, platform for the capture and detection of FLAG® fusion proteins. The ANTI-FLAG® M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigma′s GMP facility, ANTI-FLAG®P2983 high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.

Browse additional application references in our
FLAG® Literature portal.

Storage and Stability

Once plates are opened, they should be stored with desiccant at 2- 8 °C and used within two weeks.

Other Notes

The plate is supplied as a 96-well microtiter plate with clear sides and bottom.
ANTI-FLAG® M2 mouse monoclonal antibody, IgG1, is coated at a reaction volume of 200 ml/well.
The wells are pre-blocked for convenience at 275 to 300 ml/well with a complex solution containing bovine serum albumin.
The plates are specific for the FLAG epitope regardless of its placement in the fusion protein: amino-terminal, Met-amino terminal, carboxy terminal or internal. Binding of the epitope is not Ca2+ dependent.
Detection of 1 ng/well of a control fusion protein was observed in an ELISA format with p-Nitrophenyl Phosphate (pNPP) as a substrate.
Capture of 100 to 300 ng/well of a FLAG fusion protein has been demonstrated.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
F-127 is a registered trademark of BASF SE
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Shu Kachi et al.
Human gene therapy, 20(1), 31-39 (2009-01-01)
Equine infectious anemia virus (EIAV) is a nonprimate lentivirus that does not cause human disease. Subretinal injection into mice of a recombinant EIAV lentiviral vector in which lacZ is driven by a CMV promoter (EIAV CMV LacZ) resulted in rapid...
FLAG 96-well Immunoprecipitation System for High-Throughput Protein-Protein Interaction Studies
Uder, S., et al.
Molecular Biology, 4 null
Molecular dissection of the interaction between the AMPA receptor and cornichon homolog-3.
Shanks NF, Cais O, Maruo T, et al.
The Journal of Neuroscience, 34(36), 12104-12120 (2014)
Rahul Bhattacharjee et al.
Molecular biology of the cell, 31(9), 917-929 (2020-02-27)
In many organisms, positive and negative signals cooperate to position the division site for cytokinesis. In the rod-shaped fission yeast Schizosaccharomyces pombe, symmetric division is achieved through anillin/Mid1-dependent positive cues released from the central nucleus and negative signals from the...
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