Documents

P3203

Sigma-Aldrich

Anti-Protein Kinase Cβ2 antibody produced in rabbit

0.5 mg/mL, affinity isolated antibody

Synonym(s):
Anti-PKC β2
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 80 kDa

species reactivity

rat

concentration

0.5 mg/mL

application(s)

dot blot: 1:1,000
microarray: suitable
western blot: 1:8,000 using rat brain extract

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Prkcb(25023)

General description

Protein Kinase C (PKC, 76-93 kD) is a family of Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The conventional PKC isoforms (cPKC) are PKC-α, β1, β2 and γ; activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC in vivo is thought to be mediated by calpains I and II. Calpains cleave PKC in the V3 hinge region to produce two distinct fragments, one comprising the N-terminal regulatory domain (30 kD) and the other fragment containing the C-terminal kinase domain (50 kD) that is catalytically active. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C β2 antibody specifically recognises PKC β2 (80 kDa).

Specificity

In immunoblotting, the product reacts with PKC β2 (80 kDa) using rat brain extract.

Immunogen

synthetic peptide corresponding to the C-terminal region of PKC β2 (660-673).

Application

Anti-Protein Kinase C β2 antibody may be used for detection by immunoblotting at a working dilution of 1:8000 using rat brain extract. It may be used in dat blot at a working dilution of 1:1000. It is suitable for protein microarray.

Physical form

Supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Preparation Note

Purified by affinity chromatography using immobilized PKC β2 peptide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

3

Certificate of Analysis
Certificate of Origin
PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis.
Abu-Ghanem, et al.
Cancer Biology & Therapy, 6, 1375-1380 (2020)
J Garzón et al.
Neuropharmacology, 38(12), 1861-1873 (1999-12-23)
In mice whose Gi/o-protein function had been impaired by antisense 'knock-down' or pertussis toxin treatment, i.c.v. injection of myr+-Gi/o alpha subunits restored the effectiveness of beta-endorphin, morphine, DPDPE, clonidine and neurotensin to produce antinociception. Myr+-G alpha subunits of the class...
Weiqin Yin et al.
Journal of diabetes investigation, 10(3), 613-625 (2018-10-12)
Blockade or reversal the progression of diabetic nephropathy is a clinical challenge. The aim of the present study was to examine whether recombinant human glucagon-like peptide-1 (rhGLP-1) has an effect on alleviating urinary protein and urinary albumin levels in diabetic...
Domain interactions in protein kinase C.
C J Pears et al.
Journal of cell science, 100 ( Pt 4), 683-686 (1991-12-01)
S Budhiraja et al.
Fundamental & clinical pharmacology, 22(3), 231-240 (2008-05-20)
Diabetic nephropathy (DN) has emerged as the major causative pathology in patients entering end-stage renal disease (ESRD) worldwide and it is responsible for 30-40% of all ESRD cases. Treatments for DN are centered on control of hyperglycemia and blood pressure...

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