This product is intended for research use only. Unless otherwise stated this product is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
P3288
Phosphate buffer pH 7.2 at 25 °C
for hematology and histology staining techniques
Synonym(s):
phosphate buffer
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 vial | Available to ship TODAYfromMILWAUKEE | $65.10 | |
| 12 vials | Available to ship TODAYfromMILWAUKEE | $179.00 |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352107
$65.10
Available to ship TODAYDetails
Subscriptions are currently unavailable.
Ships Every 4 weeks
Quality Level
form
powder
color
white
pH
7.10-7.30
application(s)
hematology
histology
storage temp.
room temp
General description
Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).
Application
Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.
Preparation Note
Each vial will prepare 3.8 L of buffer, 8.3 mM.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 500 | Quality Level 200 |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) - |
| form powder | form powder | form powder | form liquid |
| pH 7.10-7.30 | pH 6.60-6.80 | pH 7.60-7.80 (25 °C) | pH 7.2-7.6(1x solution) |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| color white | color white | color - | color - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P3288-1VL | 04061834367604 |
| P3288-12VL | 04061834367598 |
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The current lot, 0000447969, in our lab states that this product is for R&D use only. Has this product been cleared for clinical use?
1 answer-
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could you provide me with the Wright-Giemsa protocol referenced in the question above about how to solubilize Product P3288, Phosphate Buffer pH 7.2. We need official instructions for use and the product did not come with it.
1 answer-
If this product is ordered separately, no instructions are provided. Instructions for preparing P3288 are found in the WG Instructions for Use that ship with each bottle of WG16, WG32, WG80, or WG128. Below is the information from the Reagent section of the WG procedure Instructions For Use
The phosphate buffer should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Discard the working phosphate solution if turbidity or visible bacterial growth is present. Store the working phosphate buffer solution at 2-8°C
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Once made how long is the solution good for?
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The long term stability of this product in solution has not been evaluated. Upon reconstitution, the solution should be observed for any particulate matter or turbidity which would indicate possible microbial growth. However, similar prepared solutions such as P3619, a 1M Phosphate Buffer, are not assigned an expiration date. These products are quite stable and offer a warranty of 1 year from the date of shipment.
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How to solubilize Product P3288, Phosphate Buffer pH 7.2?
1 answer-
The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C are indicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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