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P3303

Sigma-Aldrich

Endoproteinase Asp-N from Pseudomonas fragi mutant strain

suitable for protein sequencing, lyophilized powder

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1 VIAL
$277.55

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$277.55

About This Item

CAS Number:
9001-92-7
EC Number:
3.4.24.33 (BRENDA, IUBMB)
EC Number:
232-642-4
MDL number:
MFCD02253021
UNSPSC Code:
12352204
NACRES:
NA.56

$277.55

List Price$305.00Save 9%
Web-Only Promotion
Ships within 2 weeks. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
Request a Bulk Order

grade

Proteomics Grade

Quality Level

200

form

lyophilized powder

analyte chemical class(es)

amino acids

packaging

vial of 2 μg

suitability

suitable for protein sequencing

storage temp.

2-8°C

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General description

Endoproteinase Asp-N is a metallo endoprotease. It is obtained from a mutant strain of Pseudomonas fragi, which hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues.[1][2] Asp-N is used in proteomics for peptide mapping and protein sequence work due to its highly specific cleavage of peptides.[3][4]

Application

Endoproteinase Asp-N from Pseudomonas fragi mutant strain has been used for the digestion of specific proteins to prepare peptides and for the analysis of generated peptides by MS (mass spectrometry) method.[5][6][7]

pictograms

Health hazardExclamation mark
GHS08,GHS07

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Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000435712
0000435712
0000424297
0000424297
0000408865

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Functional consequences of preorganized helical structure in the intrinsically disordered cell-cycle inhibitor p27(Kip1).
Bienkiewicz EA, et al.
Biochemistry, 41, 752-752 (2002)
S R Paik et al.
The Biochemical journal, 340 ( Pt 3), 821-828 (1999-06-09)
alpha-Synuclein is a component of the abnormal protein depositions in senile plaques and Lewy bodies of Alzheimer's disease (AD) and Parkinson's disease respectively. The protein was suggested to provide a possible nucleation centre for plaque formation in AD via selective
Sarah Murray et al.
Journal of virology, 80(12), 6171-6176 (2006-05-30)
Adeno-associated virus type 2 (AAV-2) capsid proteins have eight sequence motifs that are potential sites for O- or N-linked glycosylation. Three are in prominent surface locations, close to the sites of cellular receptor attachment and to neutralizing epitopes on or
Yeast ribosomal/cytochrome c SET domain methyltransferase subfamily: identification of Rpl23ab methylation sites and recognition motifs.
Kameoka D, et al.
Journal of Biochemistry, 134, 129-135 (2003)
C E Wobus et al.
The Journal of general virology, 79 ( Pt 8), 2023-2025 (1998-08-26)
The amino acid sequence of the genome-linked viral protein (VPg) of pea enation mosaic enamovirus (PEMV) has been determined. The VPg is encoded by nt 1811-1894 within ORF1 of RNA1 downstream of the proteinase motif. Direct N terminus sequencing of
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