P3326

Platelet-Derived Growth Factor-AB human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): PDGF-AB
• MDL number: MFCD00213024
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Platelet-Derived Growth Factor-AB human, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE and N-terminal analysis)
• form: lyophilized powder
• potency: 10-100 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: 27-31 kDa
• packaging: pkg of 10 μg; pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P04085, P01127
• storage temp.: −20°C
• Gene Information: human ... PDGFA(5154), PDGFB(5155)

Description

General description

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge.[1] PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD).[2] The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.[3][4]

Application

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.[5]

Biochem/physiol Actions

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts.[6] PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair.[7] PDGF-AB isomer is found to be associated with angiogenesis and proliferation.[8] Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.[3]

Other Notes

Heterodimer of the A and B chains of PDGF disulfide linked.

Physical form

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)