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P3351

Millipore

Protein L–Agarose from Peptostreptococcus magnus

recombinant, expressed in E. coli

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NACRES:
NA.56

recombinant

expressed in E. coli

Quality Level

matrix

6% beaded agarose supplied as 50% slurry

matrix activation

cyanogen bromide

matrix attachment

amino

capacity

3-10 mg/mL binding capacity

storage temp.

2-8°C

Application

Protein L-agarose is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, phosphorylation analysis, and protein A, G and L resins. Protein L-agarose has been used to provide evidence that antineuronal antibodies may contribute to neuronal dysfunction observed in a subset of patients with neurogenic chronic intestinal pseudoobstruction. Protein L agarose has also been used to evaluate a diabody to improve protection againse a potent scorpion neurotoxin.
Protein L from Peptostreptococcus magnus binds immunoglobulins (Ig) primarily through kappa light chain interactions without interfering with the antigen binding site. Recombinant Protein L contains four Ig-binding domains.

Preparation Note

Prepared with recombinant Peptostreptococcus magnus Protein L.

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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