P3487

Sigma-Aldrich

Monoclonal Anti-PTEN antibody produced in mouse

~1 mg/mL, purified immunoglobulin, clone PTN-18, buffered aqueous solution

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PTN-18, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

application(s)

immunocytochemistry: suitable using 4% paraformaldehyde-0.5% Triton X-100 fixation
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using whole extract of rat brain

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PTEN(5728)
mouse ... Pten(19211)
rat ... Pten(50557)

General description

The PTEN (phosphatase and tensin homolog) gene is mapped to human chromosome 10q23. It shares sequence homology with protein tyrosine phosphatases and the cytoskeletal protein tensin.

Specificity

The epitope recognized by the antibody resides within amino acids 386-403 of rat PTEN.

Immunogen

synthetic peptide corresponding to the C-terminus of rat PTEN (amino acids 386-403).

Biochem/physiol Actions

The PTEN (phosphatase and tensin homolog) gene encodes a encodes a dual-specificity phosphatase that functions as a tumor suppressor by inhibiting phosphoinositide-3-kinase–protein kinase B/Akt (PI3K/PKB/Akt) signaling pathway, a crucial regulator of cell growth and survival and by negatively regulating the interactions of cell with the extracellular matrix. Mutations in this gene have been associated with several human cancers. Germline mutations in this gene have also been linked to Cowden disease (CD), Lhermitte-Duclos disease (LDD) and Bannayan-Zonana syndrome (BZS), which are characterized by increased susceptibility to tumor formation and developmental defects.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.
Li J, et al.
Science, 275(5308), 1943-1947 (1997)
Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN.
Tamura M, et al.
Science, 280(5369), 1614-1617 (1998)
Pten is essential for embryonic development and tumour suppression.
Di Cristofano A, et al.
Nature Genetics, 19(4), 348-355 (1998)
Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN.
Stambolic V, et al.
Cell, 95(1), 29-39 (1998)
Hong Lu et al.
Oncology letters, 17(1), 69-72 (2019-01-19)
The expression pattern of tumor suppressor gene phosphatase and tensin homolog deleted on chromosome ten (PTEN) and phosphatase and tensin homolog deleted on chromosome ten/phosphatidylinositol3-kinase/protein kinase B (PTEN/PI3K/AKT) cell signaling pathway in renal cell carcinoma (RCC) were investigated in children....
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