P354-05

Porcine Aortic Smooth Muscle Cells: PAOSMC (Cryovial)

• NACRES: NA.81

Properties

• biological source: Porcine aorta (normal)
• Quality Level: 100
• form: solid
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 38
• morphology: smooth muscle
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Aorta

Packaging

1 each

Application

aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation

Components

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 1st passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PAOSMC Culture Protocol.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable