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P354-05

Sigma-Aldrich

Porcine Aortic Smooth Muscle Cells: PAOSMC (Cryovial)

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UNSPSC Code:
41106514
NACRES:
NA.81

biological source

Porcine aorta (normal)

Quality Level

form

solid

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 38

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

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PAOSMC were used to investigate the effects of heparin on smooth muscle cell proliferation, specifically revealing that heparin induces MKP-1 phosphatase that dephosphorylates ERK, decreasing its activity (Blaukovitch, 2010). They were also used to show that normalization of myocardin signaling in the diseased heart by using targeted inhibition of myocardin genes by shRNA restores heart function (Torrado, 2011). Finally, PAOSMC along with Porcine Aortic Endothelial cells, have been used as controls in a study characterizing circulating endothelial progenitor cells (Allen, 2008).

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Aorta

Application

aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation

Components

Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Preparation Note

  • 1st passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PAOSMC Culture Protocol.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Nina Parmar et al.
Tissue engineering. Part C, Methods, 21(4), 404-412 (2014-09-11)
Muscle degeneration is a prevalent disease, particularly in aging societies where it has a huge impact on quality of life and incurs colossal health costs. Suitable donor sources of smooth muscle cells are limited and minimally invasive therapeutic approaches are

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