P3804

o-Phenylenediamine dihydrochloride

tablet, 5 mg substrate per tablet

• Synonym(s): Anti-HA6116, Anti-HD4, Anti-BDMR
• Linear Formula: C₆H₄(NH₂)₂·2HCl
• CAS Number: 615-28-1
• Molecular Weight: 181.06
• Beilstein: 3912045
• EC Number: 210-418-7
• MDL number: MFCD00012966
• PubChem Substance ID: 24898457
• NACRES: NA.83

Properties

• Quality Level: 200
• form: tablet
• mp: 258 °C (dec.) (lit.)
• solubility: water: 1 tablet/10 mL, clear, colorless
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Nc1ccccc1N
• InChI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChI key: RIIWUGSYXOBDMC-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Packaging

16 mg total tablet weight

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Safety Information

• Pictograms: GHS07,GHS08,GHS09
• Signal Word: Warning
• Hazard Statements: H302 + H332 - H317 - H319 - H341 - H351 - H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3