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MilliporeSigma

P3804

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

chromogenic, tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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50 TABS
$254.00
100 TABS
$411.00

$254.00

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein/REAXYS Number:
3912045
EC Number:
210-418-7
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898457
NACRES:
NA.83

Key Documents

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Product Name

o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

Quality Level

200

form

tablet

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet

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P8787

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 1 mg substrate per tablet

Sigma-Aldrich

P6662

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 2 mg substrate per tablet

Sigma-Aldrich

P6787

o-Phenylenediamine dihydrochloride

solubility

water: 1 tablet/10 mL, clear, colorless

solubility

-

solubility

water: 1 tablet/10 mL, clear, colorless

solubility

-

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used:
  • as a chromogenic substrate in indirect enzyme-linked immunosorbent assay (ELISA) to detect immunoglobin G (IgG) antibodies against certain recombinant antigens/proteins
  • as a streptavidin-horseradish peroxidase (HRP) substrate sandwich ELISA to analyze human interleukin (IL)-17A, IL-23, and IP-10
  • as a substrate in ELISA for determination of IgG-class antibodies to Mycobacterium bovis in serum

Packaging

16 mg total tablet weight

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

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Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000421541
0000421540
0000421541
0000421540
0000313049

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H G Marco et al.
Peptides, 21(9), 1313-1321 (2000-11-10)
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Arthritis research & therapy, 19(1), 139-139 (2017-06-18)
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Joanne L Casey et al.
Methods in molecular biology (Clifton, N.J.), 421, 111-124 (2008-10-02)
Large repertoires of peptides displayed on bacteriophage have been extensively used to select for ligand-binding molecules. This is a relatively straightforward process involving several cycles of selection against target molecules, and the resulting ligands can be tailored to various applications.
Katherine A Deets et al.
eLife, 10 (2021-12-24)
The innate immune system detects pathogens and initiates adaptive immune responses. Inflammasomes are central components of the innate immune system, but whether inflammasomes provide sufficient signals to activate adaptive immunity is unclear. In intestinal epithelial cells (IECs), inflammasomes activate a
Zahra Hemati et al.
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