The contents of each package are sufficient to prepare 1 Liter. To prepare, fill the vessel of choice (beaker, graduated cylinder, etc) to approximately 800 mL of distilled or deionized water. Place the vessel on a magnetic stir plate and add the appropriate-size stir bar. While stirring, add the contents of 1 package. Add the required additional volume of water to 1 liter. Allow the solution to stir until the contents are completely dissolved and no participates are present.
P3813
Phosphate buffered saline
powder, pH 7.4, for preparing 1 L solutions
Synonym(s):
PBS
Sign Into View Organizational & Contract Pricing
All Photos(1)
Select a Size
Change View
1 PKG
$9.20
10 PKG
$74.40
5 X 10 PKG
$273.00
About This Item
Product Name
Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions
Quality Level
form
powder
storage condition
dry at room temperature
color
white
pH
7.4
solubility
water: soluble
density
1.19 g/cm3 at 20—25 °C
1.22 g/cm3 at 20—25 °C
application(s)
diagnostic assay manufacturing
life science and biopharma
sample preparation
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | P38135 | P3688 | P3744 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| pH 7.4 | pH 7.4 | pH 7.4 | pH 7.6 (25 °C) |
| density 1.19 g/cm3 at 20—25 °C, 1.22 g/cm3 at 20—25 °C | density - | density - | density - |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility - |
General description
Phosphate-buffered saline (PBS) stands as a cornerstone in biological research due to its versatility and essential properties. One primary attribute is its isotonicity, closely resembling the pH, osmolarity, and ion concentrations of the human body. This makes PBS non-toxic to cells, playing a pivotal role in cell biology procedures such as washing cells, transporting tissues, and diluting cells and reagents. These applications ensure the preservation of cell viability and the maintenance of a physiological environment.
The buffering capacity of PBS, maintained through a phosphate-phosphoric acid equilibrium, provides a stable pH environment crucial for optimal enzyme activity and various biological processes. This makes PBS ideal for preparing reagents, protein dialysis, and immunohistochemistry, ensuring consistent and reliable results in experiments. Beyond cell biology, PBS may also find applications in diverse areas, including the creation of custom ELISA buffers to ensure optimal antibody-antigen interactions. Additionally, PBS can serve as a running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS is widely used for washing cells and tissues.
In summary, PBS emerges as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its significance in advancing scientific knowledge and understanding.
The buffering capacity of PBS, maintained through a phosphate-phosphoric acid equilibrium, provides a stable pH environment crucial for optimal enzyme activity and various biological processes. This makes PBS ideal for preparing reagents, protein dialysis, and immunohistochemistry, ensuring consistent and reliable results in experiments. Beyond cell biology, PBS may also find applications in diverse areas, including the creation of custom ELISA buffers to ensure optimal antibody-antigen interactions. Additionally, PBS can serve as a running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS is widely used for washing cells and tissues.
In summary, PBS emerges as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its significance in advancing scientific knowledge and understanding.
Application
It has been utilized for rinsing fixed tissues and creating a suitable buffer environment in the immunolocalization process(1)It has been employed as a diluent to assess the performance of a ELISA test kit(2)It has been applied as a buffer in a immunohistochemistry procedure(3)
Phosphate buffered saline has been used:
- in the washing solutions during immunofluorescence procedure.[1]
- for the suspension of bacterial cells.[2]
- during the fixation and immunostaining procedures.[3]
- for rinsing fixed tissues and creating a suitable buffer environment in the immunolocalization process[4]
- as a diluent to assess the performance of an ELISA test kit[5]
- as a buffer in an immunohistochemistry procedure[6][3]
Features and Benefits
- High-quality product suitable for multiple research applications
- Can be used as a buffer component in biological and biochemical applications
- Highly soluble in water
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Expliquen me como preparar la solución a partir del polvo
1 answer-
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
Does P3813 PBS (Phosphate Buffered Saline) contain calcium or magnesium?
1 answer-
The composition of P3813 PBS Phosphate Buffered Saline is considered proprietary, but the website provides the following information. When one pouch is dissolved in one liter of distilled or deionized water, it yields 0.01 M phosphate buffered saline with a pH of 7.4 at 25°C, consisting of NaCl at 0.138 M and KCl at 0.0027 M.
Helpful?
-
-
Is autoclaving necessary for PBS P3813 before conducting the presence-absence test for coliform bacteria?
1 answer-
No, phosphate buffers by themselves are generally not autoclaved due to the common occurrence of precipitate formation. Autoclaving is commonly used in microbiology, but if your test method only requires sterilization without specifying autoclaving, filter sterilization might be a better option to consider.
Helpful?
-
-
Good morning, I would like to know what PKG means in kg. I would also like information about the validity of the solution resulting from the dissolution of the solid in question. Thanks
1 answer-
PKG means package. One package is one pouch. As per its COA: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P3813-1PAK0000291289.pdf, each pouch contains 9.07-10.00 g of powder. And the pH and dissolution time are tested to validate the solution.
Helpful?
-
-
hi, what kg is in one package?
1 answer-
The amount of powder to prepare 1 liter of buffer is approximately 9.55 grams.
Helpful?
-
-
Does this PBS contain Ca and Mg?
1 answer-
No, the components of this formulation do not include calcium or magnesium.
Helpful?
-
-
What is the salinity of Phosphate buffer saline P3813?
1 answer-
As mentioned on the product detail page, the contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.
Helpful?
-
-
Is Product P3813, Phosphate buffered saline, a 1 X PBS solution?
1 answer-
Product P3813 when prepared as directed yields a 1 X PBS solution.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
