13 - Non Combustible Solids
Product P3813 when prepared as directed yields a 1 X PBS solution.
There is no calcium nor magnesium added to the powder.
The quality for unopened foil pouches stored at room temperature is guaranteed for one year from the time of receipt. The product is expected to be stable for at least 5 years.
Sigma Aldrich has not evaluated the solution stability, but sterile solutions of PBS stored at 2-8°C can be expected to be stable for several months.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Each pouch of phosphate buffered saline contains 8 g sodium chloride (e.g. Product No. S9625), 0.2 g potassium phosphate, monobasic (e.g. Product No. P5379), 1.15 g sodium phosphate, dibasic (e.g. Product No. S0876), 0.2 g potassium chloride (e.g. Product No. P4504), giving a total 9.55 g per packet. This is the standard PBS formulation.
The formulation for this phosphate buffered saline product does not contain calcium, magnesium, or sodium bicarbonate. Sigma does not assay the product for the presence of these compounds (as impurities). None of these compounds are used in the method of preparation.
Phosphate buffered saline product number P3813 is not necessarily sterile.
Each pouch of phosphate buffered saline product number P3813 can be used to prepare 1 Liter of a 1X PBS.
Phosphate buffered saline product number P3813 is not tested for RNase or DNase impurities.
Ask a Scientist here.
This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes
Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
HDM-PAMPA with the MultiScreen Permeability Filter Plate
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.