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Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

MDL number:

Quality Level






water: soluble


diagnostic assay manufacturing

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  • Phosphate buffered saline has been used in the washing solutions during immunofluorescence procedure.
  • It has been used for the suspension of bacterial cells.
  • It has been used during the fixation and immunostaining procedures.


Foil pouches


Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Is Product P3813, Phosphate buffered saline, a 1 X PBS solution?

    Product P3813 when prepared as directed yields a 1 X PBS solution.

  2. Does Product P3813, Phosphate buffered saline, contain calcium or magnesium ions?

    There is no calcium nor magnesium added to the powder.

  3. What is the shelf life of Product P3813, Phosphate buffered saline?

    The quality for unopened foil pouches stored at room temperature is guaranteed for one year from the time of receipt. The product is expected to be stable for at least 5 years.

  4. How long can solutions of Product P3813, Phosphate buffered saline, be kept once prepared?

    Sigma Aldrich has not evaluated the solution stability, but sterile solutions of PBS stored at 2-8°C can be expected to be stable for several months.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. How much is in each pouch of Product P3813, Phosphate buffered saline?

    Each pouch of phosphate buffered saline contains 8 g sodium chloride (e.g. Product No. S9625), 0.2 g potassium phosphate, monobasic (e.g. Product No. P5379), 1.15 g sodium phosphate, dibasic (e.g. Product No. S0876), 0.2 g potassium chloride (e.g. Product No. P4504), giving a total 9.55 g per packet.  This is the standard PBS formulation.

  10. Does Product P3813, Phosphate buffered saline, contain calcium, magnesium or sodium bicarbonate?

    The formulation for this phosphate buffered saline product does not contain calcium, magnesium, or sodium bicarbonate.  Sigma does not assay the product for the presence of these compounds (as impurities). None of these compounds are used in the method of preparation.

  11. Is Product P3813, Phosphate buffered saline, sterile?

    Phosphate buffered saline product number P3813 is not necessarily sterile.

  12. Is Product P3813, Phosphate buffered saline, 1X or 10X?

    Each pouch of phosphate buffered saline product number P3813 can be used to prepare 1 Liter of a 1X PBS.

  13. Is Product P3813, Phosphate buffered saline, tested for RNase or DNase impurities?

    Phosphate buffered saline product number P3813 is not tested for RNase or DNase impurities.

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Membrane Integrity Test for Lipid-PAMPA Artificial Membranes

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes


Cell Capture Imaging Reagent

Immunocytochemistry (ICC) Cell Capture Imaging Reagent and protocol for eliminating wash steps, and is highly effective in applications involving rare cells, low cell count samples, and limited access samples.

Immunofluorescence Labeling of Cells

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

HDM-PAMPA with the MultiScreen Permeability Filter Plate

HDM-PAMPA with the MultiScreen Permeability Filter Plate

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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