This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011
P3910
Protease from Bacillus licheniformis
lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A
Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
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500 MG
$638.00
5 X 500 MG
$2,400.00
$638.00
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.84
MDL number:
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form
lyophilized powder
quality
for use in Total Dietary Fiber Assay, TDF-100A
mol wt
27 kDa
application(s)
food and beverages
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Application
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
Biochem/physiol Actions
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Katja Kantanen et al.
Foods (Basel, Switzerland), 11(9) (2022-05-15)
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John Szpylka et al.
Journal of AOAC International, 95(6), 1547-1554 (2013-03-05)
The method for "Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation" was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses
Micaela Vannini et al.
Polymers, 13(7) (2021-04-04)
With the aim to fully exploit the by-products obtained after the industrial extraction of starch from sweet potatoes, a cascading approach was developed to extract high-value molecules, such as proteins and pectins, and to valorize the solid fraction, rich in
Zhaoyue Men et al.
Journal of animal science and biotechnology, 13(1), 14-14 (2022-01-17)
Dietary fiber (DF) is often eschewed in swine diet due to its anti-nutritional effects, but DF is attracting growing attention for its reproductive benefits. The objective of this study was to investigate the effects of DF intake level on oocyte
Lisete Paiva et al.
Marine drugs, 16(8) (2018-07-28)
This study evaluates, for the first time, the seasonal (winter and summer) and geographical (São Miguel⁻SMG and Santa Maria⁻SMA Islands) variability of Fucus spiralis (Fs) biochemical composition (dry weight basis) and antioxidant properties. Protein and carbohydrates presented higher values in
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QC Methods
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本試薬の比活性 ユニット/mgをご教授ください。
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This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011Helpful?
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