P4039

Sigma-Aldrich

Phospholipase C from Clostridium perfringens (C. welchii)

greener alternative

Type XIV, lyophilized powder, ≥150 units/mg protein

Synonym(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

type

Type XIV

form

lyophilized powder

specific activity

≥150 units/mg protein

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative category

Enabling

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Application

Phospholipase C from Clostridium perfringens has been used as a positive control in sandwich enzyme linked immunosorbent assay (ELISA) and ELISA.

Packaging

125 units in serum bottle

Biochem/physiol Actions

Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Unit Definition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Physical form

Lyophilized powder in buffered salts

Preparation Note

Chromatographically purified

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?

    Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.   

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

In vitro inhibitory effect of hen egg white lysozyme on Clostridium perfringens type A associated with broiler necrotic enteritis and its alpha-toxin production
Zhang G, et al.
Letters in Applied Microbiology, 42(2), 138-143 (2006)
Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry
McCourt MT, et al.
Veterinary Microbiology, 106(3-4), 259-264 (2005)
A measured approach: determining the PLCγ1 docking site on Itk using a biochemical ruler.
Ranajeet Ghose
Journal of molecular biology, 425(4), 679-682 (2012-12-26)
Hrvoje Banfic et al.
The Journal of biological chemistry, 288(3), 1717-1725 (2012-11-28)
Several studies have demonstrated the activation of phosphoinositide-specific phospholipase C (Plc) in nuclei of mammalian cells during synchronous progression through the cell cycle, but the downstream targets of Plc-generated inositol 1,4,5-trisphosphate are poorly described. Phospholipid signaling in the budding yeast...
Qian Xie et al.
Journal of molecular biology, 425(4), 683-696 (2012-12-12)
Itk (interleukin-2 inducible T cell kinase) is a non-receptor protein tyrosine kinase expressed primarily in T cells. Itk catalyzes phosphorylation on tyrosine residues within a number of its natural substrates, including the well-characterized Y783 of PLCγ1. However, the molecular mechanisms...

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