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P4112

Sigma-Aldrich

Anti-phospho-PKB (pSer473) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-phospho-Akt
MDL number:
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 56 kDa

species reactivity

rat, mouse

technique(s)

microarray: suitable
western blot: 1:1,000 using a whole extract of PDGF-treated mouse fibroblast NIH3T3 cells and a whole extract of H2O2-treated rat fibroblast Rat-1 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer473)

Gene Information

human ... AKT1(207)
mouse ... Akt1(11651)
rat ... Akt1(24185)

General description

Protein kinase B (PKB, also known as Akt) is a serine/threonine protein kinase and a key regulator of signal transduction processes. Important processes such as cell cycle, proliferation and apoptosis are regulated by PKB in response to stimuli such as growth factors, insulin and cytokines. Three isoforms of PKB have been identified, α, β and γ. PKB α is reportedly overexpressed in breast cancer cell lines, whereas PKB β is overexpressed in ovarian and pancreatic cancers. On cell stimulation by growth factors or insulin, PKB α translocates to the cell membrane, where it undergoes full activation by phosphorylation at Thr308 and Ser 473, by PDK1 and PDK2. PKB α plays a crucial role in several downstream pathways involved in glycogen synthesis, protein translation and cell proliferation. PKB is also a potent inhibitor of apoptotic cell death by suppressing BAD-induced death

Specificity

Anti-phospho PKB (pSer473) recognises PKB phosphorylated at serine 473 (56 kDa).

Immunogen

Synthetic phosphorylated peptide corresponding to (pSer473) PKBα (human, amino acids 461-477 with N-terminally added lysine), conjugated to KLH. The sequence is identical in rat, mouse, viral Akt and bovine PKBα and is highly conserved in chicken PKBα-(single amino acid substitution). The immunogen sequence is conversed to a lesser degree in PKBγ (88%) and PKBβ (64%).

Application

Anti-phospho-PKB (pSer473) antibody produced in rabbit has been used in western blotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Chang Li et al.
Oncology research, 26(2), 209-217 (2017-05-12)
Colon cancer is one of the most lethal varieties of cancer. Chemotherapy remains as one of the principal treatment approaches for colon cancer. The anticancer activity of procaine (PCA), which is a local anesthetic drug, has been explored in different
Boda Ying et al.
Oncology research, 25(9), 1463-1470 (2017-03-03)
Procaine (PCA) is a conventional chemotherapeutic agent for osteosarcoma. Recent studies have proposed that the growth-inhibitory effect of PCA is through regulation of microRNAs (miRNAs). miR-133b has been proven to be a tumor suppressor in osteosarcoma, but whether it is
Mandar Bawadekar et al.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 35(6), 441-453 (2015-02-26)
The nuclear interferon-inducible-16 (IFI16) protein acts as DNA sensor in inflammasome signaling and as viral restriction factor. Following Herpesvirus infection or UV-B treatment, IFI16 delocalizes from the nucleus to the cytoplasm and is eventually released into the extracellular milieu. Recently
M A Lawlor et al.
Journal of cell science, 114(Pt 16), 2903-2910 (2001-11-01)
The serine/threonine protein kinase PKB (also known as Akt) is thought to be a key mediator of signal transduction processes. The identification of PKB substrates and the role PKB phosphorylation plays in regulating these molecules have been a major focus
Procaine Inhibits Proliferation and Migration and Promotes Cell Apoptosis in Osteosarcoma Cells by Upregulation of MicroRNA-133b.
Ying B, et al.
Oncology Research, 25(9), 1463-1470 (2017)

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