Prepare stock solutions in 0.1 M Tris-HCl, pH 7.0. While no specific concentration was provided, calculations based on the assay procedure suggest that stock solutions should be in the range of 0.5 - 1.0 mg/ml protein. This aligns with the general recommendation for proteins of a minimum of 1 mg protein/ml. The stock solution can be stored as aliquots at -20°C and should be reasonably stable for several months.
P4234
Pyranose Oxidase from Coriolus sp.
recombinant, expressed in E. coli, ≥2.7 units/mg solid
Synonym(s):
Pyranose: Oxygen 2-Oxidoreductase
Sign Into View Organizational & Contract Pricing
Select a Size
250 UNITS
$346.00
1000 UNITS
$1,360.00
All Photos(2)
Select a Size
Change View
250 UNITS
$346.00
1000 UNITS
$1,360.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
form
powder
specific activity
≥2.7 units/mg solid
shipped in
wet ice
storage temp.
−20°C
1 of 4
This Item | P4105 | SAE0051 | G9637 |
|---|---|---|---|
| specific activity ≥2.7 units/mg solid | specific activity ≥35 units/mg protein (biuret) | specific activity ≥35 unit/mg solid | specific activity 40-80 units/mg solid, pH 8.1 |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Pyranose oxidase (P2O), a homotetrameric protein consists of a covalently bound flavin adenine dinucleotide (FAD). It is seen mostly among wood-degrading basidiomycetes.[1]
Application
Biochem/physiol Actions
Pyranose oxidase (P2O) can be used in clinical chemistry to determine 1,5-anhydro-d-glucitol marker, used for glycemic control in diabetes patients.[1]
Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide[4].
Unit Definition
One unit produces 1.0 μmol of hydrogen peroxide per minute at 37 °C, pH 7.0.
Other Notes
Contains glutamate
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
-
What is the solution stability for product P4234 after dilution?
1 answer-
Helpful?
-
-
Do you have a protocol for the activity assay used to qualify pyranose oxidase?
Do you have a protocol for the activity assay used to qualify pyranose oxidase?
1 answer-
We do not have a pre-formulated protocol available at the moment. However, for reference, you may find the following publication informative. The publication, "Limited proteolysis of pyranose 2-oxidase results in a stable and active complex" in PeerJ Materials Science 2:e7 (https://doi.org/10.7717/peerj-matsci.7), served as the basis for this assay, utilizing the production of quinoneimine dye to directly reflect the turnover rate of pyranose oxidase.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
