P4235

Anti-p53 antibody produced in sheep

fractionated antiserum, buffered aqueous solution

• MDL number: MFCD00164709
• NACRES: NA.41

Properties

• biological source: sheep
• Quality Level: 200
• conjugate: unconjugated
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 53 kDa
• species reactivity: rat, mouse, human
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: 1-10 μg/mL
• UniProt accession no.: P04637
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... TP53(7157) ; mouse ... Trp53(22059) ; rat ... Tp53(24842)

Description

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

GST fusion protein of human p53 corresponding to amino acids 1-393.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable