P4235

Sigma-Aldrich

Anti-p53 antibody produced in sheep

fractionated antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

sheep

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 53 kDa

species reactivity

mouse, human, rat

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: 1-10 μg/mL

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TP53(7157)
mouse ... Trp53(22059)
rat ... Tp53(24842)

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

GST fusion protein of human p53 corresponding to amino acids 1-393.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
ZnO nanorod-induced apoptosis in human alveolar adenocarcinoma cells via p53, survivin and bax/bcl-2 pathways: role of oxidative stress
Ahamad M
Nanomedicine: Nanotechnology, Biology, and Medicine, 7, 904-913 (2011)
Suppression of p53 potentiates chemosensitivity in nutrient?deprived cholangiocarcinoma cells via inhibition of autophagy
Fei Hui
Oncology, 14.2, 1959-1966 (2017)
Multiple myeloma involving central nervous system: high frequency of chromosome 17p13.1 (p53) deletions
Hong Chang
British Journal of Haematology, 127(3), 280?284-280?284 (2004)
Articles
p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.
Read More
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Read More

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