P4306

Platelet-Derived Growth Factor-BB human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): PDGF-BB
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00213023

Properties

• Product Name: Platelet-Derived Growth Factor-BB human, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 1.0-3.0 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: 24.3 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P01127
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... PDGFB(5155)

Description

Analysis Note

The biological activity is measured by the dose-dependent stimulation of thymidine uptake in BALB/c 3T3 cells.

Application

Platelet-Derived Growth Factor-BB human has been used in induction of DNA synthesis and proliferation in low-concentration serum.[1]

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts.[2] PDGF elicits multifunctional actions in a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes.[3][4] PDGF-BB isomer is highly expressed in tumor, mediating oncogenic pericyte recruitment and is is a key regulator of angiogenesis.[3] PDGF-BB controls bone homeostasis, repair and regeneration.[5] It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins and modulates LDL receptor binding.

General description

Platelet-Derived Growth Factor (PDGF), a cationic polypeptide, is an important serum mitogen predominantly found in cells of mesenchymal origin.[6][7] PDGF is mapped to human chromosome 7p22.3 and is expressed in smooth muscle cells, endothelial cells and macrophages.[8][7] PDGF exists as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.[3]

Other Notes

Dimer of the B chain of human PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)