P4334

Sigma-Aldrich

Anti-Protein Kinase Cα antibody produced in rabbit

whole antiserum

Synonym(s):
Anti-PKCα
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 80 kDa

contains

15 mM sodium azide

species reactivity

rat, mouse

application(s)

microarray: suitable
western blot: 1:20,000 using mouse NIH 3T3 fibroblast lysate
western blot: 1:50,000 using rat brain extract

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Prkca(18750)
rat ... Prkca(24680)

General description

Anti-Protein Kinase Cα is produced in rabbit using a synthetic peptide conjugated to keyhole limpet hemocyanin (KLH) as the immunogen. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors, and neurotransmitters.
PKC α is a conventional isoform of Protein Kinase C (76-93 kD) and a member of serine/threonine (Ser/Thr) protein kinases. It is expressed ubiquitously in tissues and is the major isoform of PKC in fibroblasts. Stimulation and overexpression of PKC α increases the growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues. It is also useful for detecting PKC α isoenzyme in brain tissue and cell extracts. Furthermore, it can be used in chemiluminescence detection systems to detect PKC α. Rabbit anti-protein kinase c α antibody reacts specifically with PKC α (80 kD) of rat brain extract or NIH3T3 mouse fibroblast lysate.

Specificity

The antibody reacts in immunoblotting with PKCα in rat brain extract. Staining is specifically inhibited with PKCα immunizing peptide (659-672).

Immunogen

Synthetic peptide corresponding to amino acids from the C-terminal variable (V5) region of rat PKC α conjugated to KLH.

Application

Anti-Protein Kinase C α has been used in immunoblotting and immunohistochemistry.
Anti-Protein Kinase Cα antibody produced in rabbit has been used in light microscopic—immunocytochemical analysis and immunofluorescent labelling.

Biochem/physiol Actions

Protein Kinase C (PKC) plays a crucial role in signal transduction leading to cellular regulation, cell growth and differentiation, oncogenesis and modulation of neurotransmission. PKC is a phospholipid dependent enzyme, activated by the lipid 1,2-diacylglycerol (DAG), an intracellular second messenger produced as a result from hydrolysis of inositol phospholipids, in response to a variety of hormones, growth factors and neurotransmitters. PKC is also the major cellular receptor for the tumor promoting phorbol esters. PKC action is mediated by binding to specific receptors for activated C-kinase (RACKs) and through the phosphorylation of several cellular substrates. Overexpression and stimulation of PKC α leads to enhanced growth rate of cells in culture. Anti-Protein Kinase C α antibody is useful for studying expression of PKC α in normal and neoplastic tissues.

Physical form

Supplied as a liquid containing 15 mM sodium azide as preservative

Storage and Stability

For continuous use, store at 2-8 °C. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

The Rod Pathway of the Microbat Retina Has Bistratified Rod Bipolar Cells and Tristratified AII Amacrine Cells
Muller B, et al.
The Journal of Neuroscience, 33(3), 1014-1023 (2013)
Protein kinase C, an elusive therapeutic target?
Mochly-Rosen D, et al.
Nature Reviews Drug Discovery, 11(12), 937-937 (2012)
Protein kinase C: structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions
Newton AC
Chemical Reviews, 101(8), 2353-2364 (2001)
K Almholt et al.
The Biochemical journal, 337 ( Pt 2), 211-218 (1999-01-12)
The alpha isoform of protein kinase C (PKCalpha) is a ubiquitous protein kinase, which, upon activation, translocates rapidly from the cytoplasm to the plasma membrane. To follow this translocation, PKCalpha was tagged with a highly fluorescent derivative of green fluorescent...
Cone photoreceptors and potential UV vision in a subterranean insectivore, the European mole
Glosmann M et al
Journal of Vision, 8, 1-12 (2008)

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