This product does not contain calcium or magnesium. The concentration of each component is as follows:
- 0.144 g/L / 1.058mM Potassium phosphate monobasic
- 9.0 g/L / 154mM Sodium chloride
- 0.528 g/L / 2.97mM Sodium phosphate dibasic dihydrate
P4474
Phosphate buffered saline
liquid, pH 7.4, sterile-filtered, suitable for cell culture
Synonym(s):
Phosphate buffered saline, PBS
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Product Name
Phosphate buffered saline, pH 7.4, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, tested
pH
7.4
Quality Level
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Related Categories
1 of 4
This Item | P2272 | P5119 | P4417 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form tablet |
| pH 7.4 | pH 7.2 | pH 7.2 (25 °C) | pH 7.2-7.6 (1 tablet/200 mL) |
| sterility sterile-filtered | sterility sterile-filtered | sterility autoclaved | sterility - |
| impurities Endotoxin, tested | impurities Endotoxin, tested | impurities - | impurities - |
General description
Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
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Is this PBS Ca²⁺/Mg²⁺-free?
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Helpful?
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Why does this PBS not contain potassium chloride?
1 answer-
Phosphate-buffered saline is a salt solution containing sodium phosphate and sodium chloride. In some formulations, it also contains potassium chloride and potassium phosphate. For example, products P4417 and P2272 contain both potassium chloride and sodium chloride.
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Is it DNAs free?
1 answer-
This product is intended for cell culture use and is not assayed for the presence of endonucleases. The specifications can be found at: https://www.sigmaaldrich.com/specification-sheet/SIGMA/P4474
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What is the conductivity of this solution?
1 answer-
Unfortunately, the conductivity is not tested for this product as this is not a specification.
Helpful?
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What is the concentration (in mM) of this solution?
1 answer-
This product is a multicomponent item. The concentration and molarity of each component is as follows:
Potassium phosphate monobasic - 0.144 g/L or 1.058mM
Sodium chloride - 9.0 g/L or 154mM
Sodium phosphate dibasic dihydrate - 0.528 g/L or 2.97mMHelpful?
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What is the composition of P4474 Phosphate buffered saline pH 7.4, liquid, sterile-filtered, suitable for cell culture ?
1 answer-
Product P4474 contains the following:
- 0.144 g/L Potassium phosphate monobasic
- 9.0 g/L Sodium chloride
- 0.528 g/L Sodium phosphate dibasic dihydrateHelpful?
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