Poly-L-lysine solution

0.01%, sterile-filtered, BioReagent, suitable for cell culture

PLL Solution
CAS Number:
MDL number:

Quality Level



product line





pkg of 50 mL




cell culture | mammalian: suitable


endotoxin, tested

shipped in


storage temp.




InChI key


Looking for similar products? Visit Product Comparison Guide

Related Categories


  • Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
  • Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
  • Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.


Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of Product P4707, Poly-L-Lysine solution?

    This product is supplied as a 0.01% w/v solution.

  4. What molecular weight Poly-L-Lysine product should I choose?

    The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kDa.

  5. Should I use Poly-L-Lysine or Poly-D-Lysine?

    Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.

  6. What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?

    For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.

  7. What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?

    A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.

  8. How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?

    Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.

  9. How should I dilute Product P4707, Poly-L-Lysine solution?

    For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution.  However, if your protocol requires a more dilute solution, you can add sterile water or sterile buffer like Phosphate Buffered Saline.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

Microparticles mediate MRP1 intercellular transfer and the re-templating of intrinsic resistance pathways.
Lu JF et al.
Pharmacological Research, 76, 77-77 (2013)
Promiscuous dimerization of the growth hormone secretagogue receptor (GHS-R1a) attenuates ghrelin-mediated signaling.
Schellekens H et al.
The Journal of Biological Chemistry, 288, 181-181 (2013)
All polymer chip for amperometric studies of transmitter release from large groups of neuronal cells.
Larsen ST and Taboryski R
Analyst, 137, 5057-5057 (2012)
Lesley T MacNeil et al.
Nature neuroscience, 12(2), 150-155 (2009-01-27)
The UNC-5 receptor mediates axon repulsion from UNC-6/netrin through UNC-40 dependent (UNC-5 + UNC-40) and independent (UNC-5 alone) signaling pathways. It has been shown that UNC-40-dependent signaling is required for long-range repulsion of UNC-6/netrin; however, the mechanisms used to regulate...
Sahar Roshanbin et al.
BMC neuroscience, 17(1), 43-43 (2016-07-02)
MCT14 (SLC16A14) is an orphan member of the monocarboxylate transporter (MCT) family, also known as the SLC16 family of secondary active transmembrane transporters. Available expression data for this transporter is limited, and in this paper we aim to characterize MCT14...
Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.
Read More
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Read More
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Read More
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Read More
Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.