P4762

Sigma-Aldrich

Papain from papaya latex

lyophilized powder, ≥10 units/mg protein

Synonym(s):
Papainase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
42030311
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥10 units/mg protein

mol wt

23.4 kDa

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

−20°C

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General description

Papain is a cysteine protease, found in Carica papaya. It is composed of a single polypeptide chain containing three disulfide bridges and a sulfhydryl group.

Application

Papain from papaya latex has been used:
  • to digest carotid artery smooth muscle cells
  • in dissecting solutions
  • to produce Fab fragments of antibodies
  • for cell dissociation since it has been shown to be more effective and less damaging with certain tissues
  • in the enzyme cocktail for dissociating human fetal retinal tissue
  • to digest chondrocytes/scaffold cultures for total DNA quantification for cell proliferation
  • as a supplement in neurobasal medium to produce retinal cell suspensions
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Packaging

25 mg in poly bottle
50, 100, 500 mg in glass bottle
1, 5 g in glass bottle
Package size based on protein content

Biochem/physiol Actions

Papain with wide specificity, cleaves peptide bonds of basic amino acids, leucine or glycine. It also hydrolyzes esters and amides. Papain is considered to digest protein substrates more broadly than the pancreatic proteases. Papain is commonly used in cell isolation procedures as it is highly efficient and less harmful than other proteases on certain tissues. The use of papain is known to yield more viable smooth muscle cells, retaining the cell′s sensitivity to stimulants. Papain papaya latex has antifungal activity against C. albicans and is a cysteine protease.
  • The pH optimum is 6.0-7.0
  • Papain hydrolyzes esters and amides
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Unit Definition

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.

Physical form

Lyophilized powder containing sodium acetate

Preparation Note

2× Crystallized

Other Notes

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

PAPAIN ENZYME: A DIGESTIVE AID
PATEL T
International Journal of Clinical and Biomedical Research, 2(1), 52-53 (2016)
Immunopanning purification and long-term culture of human retinal ganglion cells
Zhang XM, et al.
Molecular Vision, 16(1), 2867-2867 (2010)
Eco-Friendly Textile Dyeing and Finishing, 244-244 (2017)
Recent advances in chemoenzymatic peptide syntheses
Yazawa K and Numata K
Molecules (Basel), 19(9), 13755-13774 (2014)
Studies on Papaya Leaf Tea, 25-25 (2009)
Articles
Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
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