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MilliporeSigma

P4912

Phospholipase D from Streptomyces sp.

Type VII, lyophilized powder, ≥150 units/mg solid

Synonym(s):

Lecithinase D, Phosphatidylcholine phosphatidohydrolase

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250 UNITS

$314.00

500 UNITS

$565.00

1000 UNITS

$1,020.00

$314.00


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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
EC Number:
Specific activity:
≥150 units/mg solid
Biological source:
Streptomyces sp.

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biological source

Streptomyces sp.

type

Type VII

form

lyophilized powder

specific activity

≥150 units/mg solid

storage temp.

−20°C

Quality Level

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1 of 4

This Item
D4668D5884P8398
specific activity

≥150 units/mg solid

specific activity

100-250 units/mg protein

specific activity

10-25 units/mg solid

specific activity

≥100 units/mg solid

biological source

Streptomyces sp.

biological source

-

biological source

-

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Type VII

type

-

type

-

type

Type IV

Application

Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.
The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions.[1] It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.[2]

Biochem/physiol Actions

Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

Protein by biuret

Other Notes

One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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Fava beans can substitute soybean meal and rapeseed meal as protein source in diets for lactating dairy cows.
Hansen, et al.
Journal of Dairy Science, 104, 5508-5521 (2021)
Mohamed H Yaghmour et al.
Journal of lipid research, 62, 100022-100022 (2021-01-17)
Phospholipids with a choline head group are an abundant component of cellular membranes and are involved in many important biological functions. For studies on the cell biology and metabolism of these lipids, traceable analogues where propargylcholine replaces the choline head
Christine Delon et al.
The Journal of biological chemistry, 279(43), 44763-44774 (2004-08-18)
Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a
Kathryn Garner et al.
The Journal of biological chemistry, 287(38), 32263-32276 (2012-07-24)
Phosphatidylinositol transfer proteins (PITPs) are versatile proteins required for signal transduction and membrane traffic. The best characterized mammalian PITPs are the Class I PITPs, PITPα (PITPNA) and PITPβ (PITPNB), which are single domain proteins with a hydrophobic cavity that binds
Milya Davlieva et al.
Antimicrobial agents and chemotherapy, 57(1), 289-296 (2012-11-02)
Daptomycin (DAP) resistance in enterococci has been linked to mutations in genes that alter the cell envelope stress response (CESR) (liaFSR) and changes in enzymes that directly affect phospholipid homeostasis, and these changes may alter membrane composition, such as that

Questions

  1. 本製品はカルタヘナ法に該当する試薬でしょうか。また菌体除去のためのフィルターろ過はされているのでしょうか。

    1 answer
    1. The Cartagena Protocol is not applicable to this product. While the manufacuturing process is proprietary, current methods of extraction and purification would eliminate the presence of any whole, vialble organisms. This is not a sterile product. Prepared solutions may be filter-sterilized prior to use.

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