P4937
Percoll®
pH 8.5-9.5 (25 °C), suitable for cell culture
About This Item
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sterility
aseptically filled
Quality Level
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
pH
8.5-9.5 (25 °C)
density
1.13 g/mL±0.005 g/mL at 25 °C (lit.)
storage temp.
2-8°C
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General description
Application
- neonatal rat ventricular cardiomyocytes
- leucocytes from a blood sample
- peritubular myoid (PM) cells
Features and Benefits
- Low osmolality permits precise adjustment to physiological conditions without significant interference from the medium.
- Compatible with living cells and viruses and allows separation and recovery of intact, fully active systems.
- Impermeable to biological membranes, resulting in no change of buoyant density of particles during centrifugation.
- Spontaneous formation of gradient during centrifugation allows mixing of large sample volumes in the centrifuge tubes.
- Low viscosity results in rapid formation of gradients and particle separation.
- Most biological particles having sedimentation coefficient values greater than 60S can be successfully isolated in Percoll gradients.
Legal Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
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