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Key Documents

P5119

Sigma-Aldrich

Phosphate buffered saline

liquid, pH 7.2 (25 °C)

Synonym(s):

PBS

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About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

Product Name

Phosphate buffered saline, pH 7.2 (25 °C)

sterility

autoclaved

Quality Level

form

liquid

pH

7.2 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Phosphate buffered saline (PBS) is an isotonic buffer solution. This water-based salt solution is made up of disodium hydrogen phosphate, sodium chloride and, in some preparations, potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.

Application

It has also been used for the dilution of capture antibody during α-hemolysin (Hla) enzyme-linked immunosorbent assay (ELISA).
Phosphate buffered saline has been used in bis-benzimide accumulation assay.

Components

Sodium chloride, 145 mM (0.85%) in phosphate buffer, 150 mM

related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Measuring the activity of active efflux in Gram-negative bacteria
Antibiotic resistance protocols, 173-180 (2010)
ELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA)
Khodaverdian VY and Shoham M
Antimicrobial agents and chemotherapy, 6 (2013)
Experimental? review? of? cobalt? induced cardiomyopathy
Zadnipryany I, et al.
Russian Open Medical Journal, 6(1), 103-103 (2017)
Daniela Michlmayr et al.
Cell reports, 31(4), 107569-107569 (2020-04-30)
Zika virus (ZIKV) is an emerging, mosquito-borne flavivirus responsible for recent epidemics across the Americas, and it is closely related to dengue virus (DENV). Here, we study samples from 46 DENV-naive and 43 DENV-immune patients with RT-PCR-confirmed ZIKV infection at
Beatriz del Rio et al.
Clinical and vaccine immunology : CVI, 15(9), 1429-1435 (2008-07-18)
Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as Lactobacillus spp.

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