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P5368

Sigma-Aldrich

Phosphate buffered saline

BioPerformance Certified, pH 7.4

Synonym(s):
PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

Quality Level

grade

BioPerformance Certified
for molecular biology

form

powder

impurities

BioBurden, tested
endotoxin, tested

pH

7.4

cation traces

Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm

Featured Industry

Diagnostic Assay Manufacturing

foreign activity

DNase, RNase, Protease, none detected

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Packaging

Foil Pouch

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?

    No.  The complete formulation is shown in the product listing.  When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.

  4. If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?

    No.  To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.

  5. What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?

    When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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