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P5380

Sigma-Aldrich

Protease from Bacillus licheniformis

Type VIII, lyophilized powder, 7-15 units/mg solid

Synonym(s):

Proteinase from Bacillus licheniformis, Subtilisin A

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$71.80
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$138.00
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$229.00
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$673.00

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Estimated to ship onJuly 31, 2025FromMILWAUKEE


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25 MG
$71.80
100 MG
$138.00
250 MG
$229.00
1 G
$673.00

About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

$71.80


Estimated to ship onJuly 31, 2025FromMILWAUKEE


Request a Bulk Order

biological source

Bacillus licheniformis

Quality Level

type

Type VIII

form

lyophilized powder

specific activity

7-15 units/mg solid

mol wt

27 kDa

purified by

crystallization

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H2O: soluble, colorless

foreign activity

DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid

storage temp.

−20°C

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1 of 4

This Item
P4860P5459P8038
biological source

Bacillus licheniformis

biological source

-

biological source

Bacillus licheniformis

biological source

-

specific activity

7-15 units/mg solid

specific activity

≥2.4 U/g

specific activity

≥7 units/mg protein (biuret)

specific activity

≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid

form

lyophilized powder

form

aqueous solution

form

glycerol solution (50%)

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

27 kDa

mol wt

-

mol wt

-

mol wt

27 kDa

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

solubility

-

solubility

0.85% sodium chloride: soluble, clear (Diluted), H2O: miscible

solubility

-

General description

The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination.[1] It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.[2]
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Questions

  1. Hi  How I can mix my enzyme with water?

    1 answer
    1. This product is soluble in water at normal usage concentrations.

      Kindly review the technical data sheet for concentration suggestions available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/194/864/p5380pis-ms.pdf

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