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P5412

o-Phenylenediamine

peroxidase substrate, chromogenic, tablet

Synonym(s):

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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50 TABS

$288.00

100 TABS

$531.00

$288.00

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About This Item

Empirical Formula (Hill Notation):
C6H8N2
CAS Number:
95-54-5
Molecular Weight:
108.14
Beilstein/REAXYS Number:
606074
EC Number:
202-430-6
MDL number:
MFCD00007721
UNSPSC Code:
12352204
PubChem Substance ID:
24898638
NACRES:
NA.83

Key Documents

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Product Name

o-Phenylenediamine, tablet, 20 mg substrate per tablet

vapor density

3.7 (vs air)

Quality Level

200

vapor pressure

0.01 mmHg ( 25 °C)

form

tablet

bp

256-258 °C

mp

100-102 °C

fluorescence

λex 337 nm; λem 417 nm (after derivatization)

storage temp.

2-8°C

SMILES string

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI key

GEYOCULIXLDCMW-UHFFFAOYSA-N

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This Item
P9029P23938694975
o-Phenylenediamine tablet, 20 mg substrate per tablet

Sigma-Aldrich

P5412

o-Phenylenediamine

o-Phenylenediamine Peroxidase substrate, ≥98.0%, powder

Sigma-Aldrich

P9029

o-Phenylenediamine

o-Phenylenediamine flaked, 99.5%

Sigma-Aldrich

P23938

o-Phenylenediamine

o-Phenylenediamine sublimed, ≥99%

Sigma-Aldrich

694975

o-Phenylenediamine

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

tablet

form

powder

form

powder

form

sublimed

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

-

fluorescence

λex 337 nm; λem 417 nm (after derivatization)

fluorescence

λex 337 nm; λem 417 nm (Pyruvic acid derivative)

fluorescence

-

fluorescence

-

vapor density

3.7 (vs air)

vapor density

3.7 (vs air)

vapor density

3.7 (vs air)

vapor density

3.7 (vs air)

vapor pressure

0.01 mmHg ( 25 °C)

vapor pressure

0.01 mmHg ( 25 °C)

vapor pressure

0.01 mmHg ( 25 °C)

vapor pressure

0.01 mmHg ( 25 °C)

Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
o-Phenylenediamine has been used to detect transforming growth factor (TGF-β)[1] and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).[2]

Biochem/physiol Actions

o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.[3]

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

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Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

276.8 °F - closed cup

flash_point_c

136 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
0000402182
0000402181
0000402181
0000402182
0000368793

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Application of o-phenylenediamine as a fluorogenic substrate in peroxidase-mediated enzyme-linked immunosorbent assay
Mekler VM and Bystryak SM
Analytica Chimica Acta, 264(2), 359-363 (1992)
Interleukin-13 Regulates Secretion of the Tumor Growth Factor-beta Superfamily Cytokine Activin A in Allergic Airway Inflammation
Hardy CL, et al.
American Journal of Respiratory Cell and Molecular Biology, 42(6), 667-675 (2010)
Receptor-mediated abeta amyloid antibody targeting to Alzheimer?s disease mouse brain
Zhou QH, et al.
Molecular Pharmaceutics, 8(1), 280-285 (2010)
Jonas R Knudsen et al.
International journal of molecular sciences, 21(7) (2020-04-02)
The small molecule kinase inhibitor SBI-0206965 was originally described as a specific inhibitor of ULK1/2. More recently, it was reported to effectively inhibit AMPK and several studies now report its use as an AMPK inhibitor. Currently, we investigated the specificity
Harrison D Stierwalt et al.
Physiological reports, 6(24), e13956-e13956 (2018-12-29)
Ras-related C3 botulinum toxin substrate 1 (Rac1) is required for normal insulin-stimulated glucose transport in skeletal muscle and evidence indicates Rac1 may be negatively regulated by lipids. We investigated if insulin-stimulated activation of Rac1 (i.e., Rac1-GTP binding) is impaired by
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