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  • P5511
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P5511

Sigma-Aldrich

Protein Kinase A from bovine heart

≥0.8 units/μg protein, lyophilized powder

Synonym(s):
Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
32160410

form

lyophilized powder

Quality Level

specific activity

≥0.8 units/μg protein

composition

Protein, ≥70%

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
P6062N2635L2625
form

lyophilized powder

form

powder

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

UniProt accession no.

P00517, P05131

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P19858, Q5E9B1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Gene Information

cow ... PRKACA(282322), PRKACB(282323)

Gene Information

-

Gene Information

-

Gene Information

cow ... LDHA(281274), LDHB(281275)

specific activity

≥0.8 units/μg protein

specific activity

-

specific activity

≥1,000 units/mg protein (biuret)

specific activity

≥500 units/mg protein

General description

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

Application

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

Biochem/physiol Actions

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Packaging

Package size based on protein content

Unit Definition

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

Physical form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Preparation Note

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Analysis Note

Protein determined by biuret.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

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