P5511

Protein Kinase A from bovine heart

≥0.8 units/μg protein, lyophilized powder

• Synonym(s): Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
• CAS Number: 9026-43-1
• Enzyme Commission number: 2.7.1.37 (BRENDA, IUBMB)
• MDL number: MFCD00132121
• eCl@ss: 32160410

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥0.8 units/μg protein
• composition: Protein, ≥70%
• UniProt accession no.: P00517; P05131
• storage temp.: −20°C
• Gene Information: cow ... PRKACA(282322) , PRKACB(282323)

Description

General description

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

Application

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.

Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

Biochem/physiol Actions

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.

Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Packaging

Package size based on protein content

Unit Definition

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

Physical form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Preparation Note

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves