All Photos(1)



Protein Kinase A from bovine heart

≥0.8 units/μg protein, lyophilized powder

Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
CAS Number:
Enzyme Commission number:
MDL number:


lyophilized powder

Quality Level

specific activity

≥0.8 units/μg protein


Protein, ≥70%

UniProt accession no.

storage temp.


Gene Information

Looking for similar products? Visit Product Comparison Guide

General description

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.


Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.


1, 5 mg in glass bottle
10 mg in poly bottle
Package size based on protein content

Biochem/physiol Actions

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Unit Definition

One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.

Physical form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Preparation Note

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Analysis Note

Protein determined by biuret.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents


Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service