P5511

Sigma-Aldrich

Protein Kinase A from bovine heart

≥0.4 units/μg protein, lyophilized powder

Synonym(s):
Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
32160410

form

lyophilized powder

Quality Level

specific activity

≥0.4 units/μg protein

composition

Protein, ≥70%

storage temp.

−20°C

Gene Information

cow ... PRKACA(282322), PRKACB(282323)

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General description

Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.

Application

Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.

Packaging

10 mg in poly bottle
1, 5 mg in glass bottle
Package size based on protein content

Biochem/physiol Actions

Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.

Unit Definition

One unit will transfer 1.0 picomole phosphate from γ-32P-ATP to hydrolyzed and partially dephosphorylated casein per min at pH 6.5 at 30 °C in the presence of cyclic AMP.

Physical form

Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.

Preparation Note

Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).

Analysis Note

Protein determined by biuret.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Alkylation of Staurosporine to Derive a Kinase Probe for Fluorescence Applications
Dr. Alexandar
Chemistry of Materials (2016)
Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
Journal of Bioenergetics and Biomembranes (2017)
Ilse A E Bollen et al.
Frontiers in physiology, 8, 1103-1103 (2018-01-10)
Dilated cardiomyopathy amongst children (pediatric cardiomyopathy, pediatric CM) is associated with a high morbidity and mortality. Because little is known about the pathophysiology of pediatric CM, treatment is largely based on adult heart failure therapy. The reason for high morbidity...
Signaling the signal: Protein Kinase a (PKA) inhibition by insulin-formed H2O2 and reactivation by thioredoxin
de Pina MZ, et al.
The Journal of Biological Chemistry (2008)
Probes of the mitochondrial cAMP-dependent protein kinase
Shell JR and Lawrence DS
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1834(7), 1359-1363 (2013)

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