The enzyme can be solubilized in 10 mM Tris-HCl, pH 7.4, containing 144 mM NaCl and 0.05% BSA. Store this product at –20 °C. The lyophilized product, when stored properly, retains activity for at least 2 years. A preparation of this enzyme in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0, and 10 mM EDTA retains activity at 2–8 °C for at least 3 years. Please see the link below to review additional product information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/218/p5542dat.pdf
P5542
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus
lyophilized powder, ≥1,000 units/mg protein (Lowry)
Synonym(s):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
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5 UNITS
$305.00
25 UNITS
$1,040.00
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
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Product Name
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus, lyophilized powder, ≥1,000 units/mg protein (Lowry)
form
lyophilized powder
specific activity
≥1,000 units/mg protein (Lowry)
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein
storage temp.
−20°C
Quality Level
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
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1 of 4
This Item | P8804 | 525186 | P6621 |
|---|---|---|---|
| form lyophilized powder | form buffered aqueous glycerol solution | form solid | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| specific activity ≥1,000 units/mg protein (Lowry) | specific activity ≥1,000 units/mg protein (Lowry) | specific activity ≥50 units/mg dry wt. | specific activity ≥200 units/mg protein |
| foreign activity Phopholipase C (lecithinase) ≤1 units/mg protein | foreign activity Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein | foreign activity - | foreign activity - |
| Gene Information Bacillus cereus E33L ... BCZK3513(3026815) | Gene Information Bacillus cereus E33L ... BCZK3513(3026815) | Gene Information - | Gene Information Bacillus cereus ATCC 10987 ... plC(2749087) |
Analysis Note
Acetylcholinesterase is measured according to Ellman, et al.
Application
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane.[1][2] It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.[3]
Biochem/physiol Actions
Used for the release of GPI anchored proteins from the membrane.
General description
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.[4]
Other Notes
One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).
Physical form
Contains phosphate buffer salts, EDTA and stabilizer
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Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.
Sartori MJ
Tropical Medicine & International Health, 8(9), 832-839 (2003)
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW
The Embo Journal, 14(16), 3855-3863 (1995)
Danielle K Bailey et al.
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Iron and other transition metals, such as copper and manganese, are essential for supporting brain function, yet over-accumulation is cytotoxic. This over-accumulation of metals, particularly iron, is common to several neurological disorders; these include Alzheimer's disease, Parkinson's disease, Friedrich's ataxia
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Brameshuber M
The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
Helen Neuert et al.
Development (Cambridge, England), 147(2) (2019-12-22)
The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily
Articles
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
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