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  • P5542
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P5542

Sigma-Aldrich

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

All Photos(3)
Synonym(s):
1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
CAS Number:
37288-19-0
Enzyme Commission number:
4.6.1.13 (BRENDA, IUBMB)
EC Number:
232-638-2
MDL number:
MFCD00131896
NACRES:
NA.32

form

lyophilized powder

Quality Level

300

specific activity

≥1,000 units/mg protein (Lowry)

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein

storage temp.

−20°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

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Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus lyophilized powder, ≥1,000 units/mg protein (Lowry)

Sigma-Aldrich

P5542

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

Sigma-Aldrich

P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Phospholipase C from Clostridium perfringens (C. welchii) Type XIV, lyophilized powder, ≥150 units/mg protein

Sigma-Aldrich

P4039

Phospholipase C from Clostridium perfringens (C. welchii)

Phospholipase C from Clostridium perfringens (C. welchii) Type I, lyophilized powder, 10-50 units/mg protein

Sigma-Aldrich

P7633

Phospholipase C from Clostridium perfringens (C. welchii)

form

lyophilized powder

form

buffered aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

≥150 units/mg protein

specific activity

10-50 units/mg protein

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

foreign activity

-

foreign activity

-

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

Gene Information

Clostridium perfringens str. 13 ... plc(988262)

General description

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Unit Definition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physical form

Contains phosphate buffer salts, EDTA and stabilizer

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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