P5622

Sigma-Aldrich

Anti-Pen-2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 10 kDa

species reactivity

human

application(s)

microarray: suitable
western blot: 3-6 μg/mL using whole extract of human kidney 293 cells expressing human Pen-2

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... PSENEN(55851)
mouse ... Psen2(19165)

General description

Presenilin enhancer γ-secretase subunit (Pen-2/ PSENEN) gene, with four exons spanning 1.4 kb, is mapped to human chromosome 19q13.12. Pen-2 is an integral membrane protein with 101 amino acids. The protein is characterized with two transmembrane domains and a cytosolic loop domain, with the C- and N-termini facing the lumen of the endoplasmic reticulum.

Specificity

Anti-PEN2 antibody recognizes Pen-2 (10 kDa).

Immunogen

synthetic peptide encoding amino acids 86-101 located at the C-terminus of human pen-2, conjugated to KLH. This sequence is identical in mouse pen-2.

Application

Anti-Pen-2 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Pen-2 is a critical component of the presenilin (PS)1/γ-secretase and PS2/γsecretase complexes. siRNA interference mediated down regulation of pen-2 is due to reduced PS levels, impaired nicastrin (Nct) maturation and deficient γ secretase complex formation. Pen-2 preferentially interacts with PS1 and induce PS1 endoproteolysis. In addition, it also stabilizes PS fragment heterodimer within the γ-secretase complex subsequent to PS endoproteolysis. PEN-2 plays a vital role in γ-secretase complex maturation in the absence of PS endoproteolysis. Mutation in the PEN-2 gene leads to the development of familial comedones syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Dopamine D2 receptor and $\beta$-arrestin 2 mediate Amyloid-$\beta$ elevation induced by anti-parkinson?s disease drugs, levodopa and piribedil, in neuronal cells
Lu J, et al.
PLoS ONE, 12(3), e0173240-e0173240 (2017)
Membrane topology of $\gamma$-secretase component PEN-2
Crystal AS, et al.
The Journal of biological chemistry, 278(22), 20117-20123 (2003)
PEN-2 is an integral component of the $\gamma$-secretase complex required for coordinated expression of presenilin and nicastrin
Steiner H, et al.
The Journal of biological chemistry, 277(42), 39062-39065 (2002)
Requirement of PEN-2 for stabilization of the presenilin N-/C-terminal fragment heterodimer within the $\gamma$-secretase complex
Prokop S, et al.
The Journal of Biological Chemistry, 279(22), 23255-23261 (2004)
A frameshift mutation in PEN-2 causes familial comedones syndrome
Panmontha W, et al.
Dermatology (Basel, Switzerland), 231(1), 77-81 (2015)
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