Presenilin enhancer γ-secretase subunit (Pen-2/ PSENEN) gene, with four exons spanning 1.4 kb, is mapped to human chromosome 19q13.12. Pen-2 is an integral membrane protein with 101 amino acids. The protein is characterized with two transmembrane domains and a cytosolic loop domain, with the C- and N-termini facing the lumen of the endoplasmic reticulum.
Anti-PEN2 antibody recognizes Pen-2 (10 kDa).
synthetic peptide encoding amino acids 86-101 located at the C-terminus of human pen-2, conjugated to KLH. This sequence is identical in mouse pen-2.
Anti-Pen-2 antibody produced in rabbit has been used in immunoblotting.
Pen-2 is a critical component of the presenilin (PS)1/γ-secretase and PS2/γsecretase complexes. siRNA interference mediated down regulation of pen-2 is due to reduced PS levels, impaired nicastrin (Nct) maturation and deficient γ secretase complex formation. Pen-2 preferentially interacts with PS1 and induce PS1 endoproteolysis. In addition, it also stabilizes PS fragment heterodimer within the γ-secretase complex subsequent to PS endoproteolysis. PEN-2 plays a vital role in γ-secretase complex maturation in the absence of PS endoproteolysis. Mutation in the PEN-2 gene leads to the development of familial comedones syndrome.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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